电离辐射诱发人外周血miRNA-150表达水平的改变及意义  

Ionizing radiation induced miRNA-150 changes in peripheral blood

作　　者：侯殿俊[1] 马娅[1] 李洁清[1] 毛雪松[1] 朱伟[1] HOU Dianjun;MA Ya;LI Jieqing;MAO Xuesong;ZHU Wei(Institute of Radiation Medicine Shandong Academy of Medical Sciences Shandong First Medical University,Jinan 250062 China)

机构地区：[1]山东省医学科学院放射医学研究所山东第一医科大学,山东济南250062

出　　处：《中国辐射卫生》2019年第6期606-608,613,共4页Chinese Journal of Radiological Health

基　　金：山东省医学科学院医药卫生科技创新工程(2017-12-A)。

摘　　要：目的通过实时定量PCR技术检测不同剂量照射引起人外周血miRNA-150表达的差异,从而探讨miRNA-150在机体辐射损伤中的意义。方法将采集的静脉血分别给予0.5、1、2、4、8 Gy剂量照射,并设置对照组。照射后将血样接种于含10%小牛血清的RPMI1640培养瓶中,置于37℃恒温培养箱中培养,利用实时定量PCR技术检测照射8、24 h后miR-150的含量变化;采用tbools软件进行miR-150序列比对,Starbase数据库预测miR-150靶基因。结果miR-150在0.5、1、2、4、8 Gy照射后8 h后2-△△Ct值分别为0.66、0.68、0.74、0.75、0.63,与未照射组相比,差异有统计学意义;照射24 h后2-△△Ct值分别为0.94、0.95、0.71、0.59、0.67,较8 h均出现了不同程度的表达下调,与未照射组相比,差异有统计学意义;人与小鼠miR-150-5p序列一致,miR-150-3p种子序列区存在单一碱基差异,靶基因预测表明BASP1、MAP3K12和ZBTB4基因为人miR-150靶向基因的可能性最高。结论照射后外周血中miR-150差异表达与辐射损伤有关,可成为预测辐射损伤的潜在生物标志物。Objective To study the differential regulations of miRNA-150 in human peripheral blood after ionizing radiation.Methods Venous blood was collected and exposed to 0.5 Gy、1 Gy、2 Gy、4 Gy、8 Gy X-ray and set a control group at the same time.Blood samples were inoculated in culture bottles containing 10%calf serum and cultured in 37℃incubators after radiation.miRNA-150 changes were detected after 8 and 24 hours post radiation by PCR.Sequence alignment for miRNA150 use tbools software.Predict the target gene of miRNA150 use Starbase datebase.Results After 8 h with 0.5 Gy、1 Gy、2 Gy、4 Gy、8 Gy radiation,miRNA-150 expression levels were 0.66、0.68、0.74、0.75、0.63,compared with the unirradiated group,the results were statistically significant;miRNA-150 expression levels were 0.94、0.95、0.71、0.59、0.67 after 24 h,the expression was downregulated compared to 8 h,compared with the unirradiated group,the results were statistically significant.Human and mouse have the same sequence in miRNA-150-5p.There is a single base difference in the Seed sequence region of miRNA-150-3p.The target gene prediction shows that there is a highest probability of BASP1,MAP3K12 and ZBTB4 as the target genes of miRNA-150 for human.Conclusion The expression of miRNA-150 in peripheral blood after radiation is related to radiation injury and has a potential serum marker for radiation injury.

关 键 词：电离辐射 miRNA-150 生物标志物 辐射损伤 

分 类 号：Q691[生物学—生物物理学]