铝毒胁迫下甘蓝型油菜种子萌发期相关性状的QTL定位  

QTL mapping of seed germination-related traits in Brassica napus L.under aluminum toxicity stress

作　　者：王瑞莉 王刘艳 叶桑 郜欢欢 雷维 吴家怡 袁芳 孟丽姣 唐章林[1] 李加纳[1] 周清元[1] 崔翠[1] WANG Rui-Li;WANG Liu-Yan;YE Sang;Gao Huan-Huan;LEI Wei;WU Jia-Yi;YUAN Fang;MENG Li-Jiao;TANG Zhang-Lin;LI Jia-Na;ZHOU Qing-Yuan;CUI Cui(College of Agronomy and Biotechnology,Southwestern University,Chongqing 400715,China)

机构地区：[1]西南大学农学与生物科技学院,重庆400715

出　　处：《作物学报》2020年第6期832-843,共12页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2018YFD0100500);国家现代农业产业技术体系建设专项(CARS-12);重庆市技术创新与应用发展(cstc2019jscx-msxmX0383)项目资助。

摘　　要：随着土壤酸化的日益加重,铝毒已成为影响作物种子萌发质量以及作物产量的重要胁迫因子之一。作物耐铝相关性状的QTL定位和候选基因筛选已有许多报道,但铝胁迫下甘蓝型油菜萌发期相关性状的QTL定位报道较少。本文以80μg mL^-1的铝胁迫浓度处理重组自交系(10D130×中双11号)群体进行种子萌发试验,处理3 d时调查发芽势,7 d时调查发芽率,测定其根长、芽长和干重,并计算各性状相对值。基于6K SNP芯片,结合高密度遗传连锁图谱对油菜萌发期的5个性状进行QTL定位,共检测到23个QTL。其中与相对发芽势、相对发芽率、相对根长、相对芽长和相对干重相关的QTL分别有9个、1个、4个、5个和4个,覆盖了A、C基因组,LOD值介于3.00~5.26,可解释的表型变异为7.70%~13.10%。根据各QTL置信区间序列筛选,与铝胁迫相关的候选基因共30个。ALMT1基因和MATE基因与有机酸的合成和分泌有关,主要通过苹果酸、柠檬酸和草酸等有机酸的分泌来增强植物的耐铝性;STOP1基因、NAC基因和RAP2.4基因均属于转录激活因子,通过诱导耐铝基因的表达增强植株的抗性;ABC转运蛋白、膜蛋白转运体、GDSL脂肪酶通过减少有毒物质在质膜上的积累或将有毒物质排出体外等途径增强植物的耐铝性;过氧化物酶和细胞色素P450均属于氧化胁迫相关基因,具有防止植物细胞氧化损伤、抵御逆境胁迫的功能;另外,还有部分编码逆境蛋白的基因,均在各种胁迫反应中起重要作用。本研究的结果将为培育耐铝油菜品种及后续基因的功能研究提供理论依据。With the aggravation of soil acidification,aluminum toxicity has become one of the important stress factors that affect crop seed germination quality and crop yield.There have been many reports on QTL mapping and candidate gene screening for aluminum tolerance related traits in crops,but few reports on QTL mapping for aluminum tolerance during germination in Brassica napus L.The seed germination test was conducted on the recombinant inbred line population treated with 80μg mL^-1 aluminum solution and with distilled water as control.Germination potential was investigated at 3 d,germination rate was investigated at 7 d,root length,bud length and dry weight were measured,and the relative values of various characters were calculated.Based on 6 K SNP chip and combined with high density genetic linkage map,we detected 23 QTLs for five traits in rape germination period.QTLs for relative germination potential,relative germination rate,relative root length,relative bud length and relative dry weight were 9,1,4,5,and 4 respectively,covering A and C genomes,with LOD values from 3.00 to 5.26,and interpretable phenotypic variation from 7.70%to 13.10%.According to the confidence interval sequence of each QTL,30 candidate genes related to aluminum stress were screened.ALMT1 gene and MATE gene are related to the synthesis and secretion of organic acids,which mainly enhance the aluminum tolerance of plants through the secretion of organic acids such as malic acid,citric acid and oxalic acid.STOP1 gene,NAC gene and RAP2.4 gene all belong to transcription activation factors,which enhance the resistance of plants by inducing the expression of aluminum tolerance genes.ABC transporter,membrane protein transporter,GDSL lipase enhance aluminum tolerance of plants by reducing accumulation of toxic substances on plasma membrane or discharging toxic substances out of body.Peroxidase and cytochrome P450 are genes related to oxidative stress,which have the functions of preventing oxidative damage of plant cells and resisting stress.At the s

关 键 词：甘蓝型油菜 萌发期 单核苷酸多态性 铝胁迫 QTL 候选基因筛选 

分 类 号：X503.231[环境科学与工程—环境工程] S565.4[农业科学—作物学]