覆盖作物不同利用方式对猕猴桃园土壤微生物群落结构的影响  

Effects of different cover crop treatments on soil microbial community composition in kiwifruit orchard

作　　者：李青梅 张玲玲 赵建宁 张艳军 刘红梅 王华玲 王慧 杨殿林 张凡[4] 翁昌明 LI Qing-mei;ZHANG Ling-ling;ZHAO Jian-ning;ZHANG Yan-jun;LIU Hong-mei;WANG Hua-ling;WANG Hui;YANG Dian-lin;ZHANG Fan;WENG Chang-ming(Agro-Environment Protection Institute,Ministry of Agriculture and Rural Affairs,Tianjin 300191,China;Key Laboratory of Origin Environmental Pollution Prevention and Control,Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agro-environment and Agro-product Safety,Tianjin 300191,China;Shiyan Institute of Economic Crop Research,Shiyan 442714,China;Crop Cultivation Institute,Shiyan Academy of Agricultural Sciences,Shiyan 442000,China;Shiyan Animal Husbandry Technology Promotion Station,Shi yan 442000,China)

机构地区：[1]农业农村部环境保护科研监测所,天津300191 [2]农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室,天津300191 [3]十堰市经济作物研究所,湖北十堰442714 [4]十堰市农业科学院作物栽培研究所,湖北十堰442000 [5]十堰市畜牧技术推广站,湖北十堰442000

出　　处：《农业资源与环境学报》2020年第3期319-325,共7页Journal of Agricultural Resources and Environment

基　　金：中国农业科学院科技创新工程协同创新任务(CAAS-XTCX2016015)。

摘　　要：为探讨覆盖作物不同利用方式对猕猴桃园土壤微生物群落结构的影响,通过磷脂脂肪酸(PLFA)方法,研究覆盖作物处理对猕猴桃园土壤微生物群落结构的影响。试验设置3个处理,分别为种植覆盖作物+覆盖作物刈割后留在土壤表面自然腐解(T1)、种植覆盖作物+覆盖作物刈割后从园中清除(T2)和清耕对照(CK)。结果表明,T1、T2处理土壤微生物PLFAs总量和微生物量碳、氮均显著高于CK(P<0.05);土壤pH和碳氮比是影响猕猴桃园土壤微生物群落的主要环境因子,pH与土壤微生物PLFAs总量呈极显著正相关(P<0.01),与细菌PLFAs量、革兰氏阴性菌PLFAs量呈显著正相关(P<0.05),碳氮比与革兰氏阳性菌PLFAs量/革兰氏阴性菌PLFAs量呈极显著正相关(P<0.01)。研究表明,猕猴桃园种植覆盖作物改变了土壤环境因子,影响土壤微生物群落结构组成。研究可为丹江口水源涵养区果园生态管理模式的探索提供理论依据。In order to provide a theoretical basis for ecological management model in an orchard, we investigated the characteristics of soil microbial community composition under cover crop treatments in a kiwifruit orchard in the water source conservation area of Danjiangkou.We used the phospholipid fatty acid(PLFA)method to analyze the effects of different treatments(T1:Cover crop left on soil after cutting;T2:Cover crop and removed after cutting;CK:Clean tillage)on soil microbial community structure. The results showed that the total PLFAs, microbial biomass carbon, and nitrogen of cover crop treatments(T1 and T2)were significantly higher than that of CK(P<0.05).Soil microbial community was influenced mainly by soil pH and carbon/nitrogen ratio. Soil pH was positively correlated with total PLFAs,bacteria PLFAs, Gram-negative bacteria PLFAs. And there was a positive relationship between Gram-positive bacteria PLFAs/Gram-negative bacteria PLFAs ratio and carbon/nitrogen ratio. In summary, intercropping cover crop in kiwifruit orchard changed soil environmental factors and impacted soil microbial community composition.

关 键 词：覆盖作物 土壤 磷脂脂肪酸 理化因子 生态果园 

分 类 号：Q938.1[生物学—微生物学] S663.4[农业科学—果树学]