一株烟草肠杆菌(Enterobacter tabaci)S4菌株的鉴定及其效果测定  

Identification and evaluation of Enterobacter tabaci strain S4

作　　者：王威 THINZAR Toe 张亚[2] 张克诚[1] 姜明国[3] 张维 施李鸣[1] 葛蓓孛[1] WANG Wei;THINZAR Toe;ZHANG Ya;ZHANG Ke-cheng;JIANG Ming-guo;ZHANG Wei;SHI Li-ming;GE Bei-bei(Institute of Plant Protection,Chinese Academy of Agricultural Science,Beijing 100193,China;College of Plant Protection,Hunan Ag ricultural University,Changsha 410128,China;Guangxi University for Nationalities,Nanning 530006,China;Biotechnology Research Center,Department of Research and Innovation,Kyaukse 05152,Myanmar)

机构地区：[1]中国农业科学院植物保护研究所,北京100193 [2]湖南农业大学植物保护学院,长沙410128 [3]广西民族大学,南宁530006 [4]缅甸生物技术研究中心创新技术研究所,皎施05152

出　　处：《农业资源与环境学报》2020年第3期407-412,共6页Journal of Agricultural Resources and Environment

基　　金：中国农业科学院科技创新工程协同创新任务(CAAS-XTCT2016015);广西科技重大专项(桂科AA18242026);广西科技基地和人才专项(桂科AD18126005)。

摘　　要：为筛选出一株具有微生物源植物调节剂功能的细菌,并探讨其潜在的生物功能,利用平板涂布法以及16S rRNA鉴定技术对菌株进行分离、鉴定;同时使用该菌株发酵液对番茄的种子和幼苗分别进行浸种和灌根处理,并进行农艺指标测定。结果表明:经鉴定该菌株为烟草肠杆菌,其发酵液对番茄种子的萌发、幼苗的生长具有显著的促进作用;发酵液浓度为1×104cfu·mL-1时促生效果最好,与对照相比,根长、株高、干质量和鲜质量分别增加63.42%、46.17%、150.00%和144.83%。研究结果可为该菌株开发为微生物肥料或微生物源植物生长调节剂提供技术支持。To screen for a bacterial strain with plant growth promotion functions and to explore its potential biological functions,strains were isolated and identified via the plate coating method and 16S rRNA identification technology in the present study.Tomato seeds and seedlings were treated using fermentation broth and biological agronomic indexes were determined.The strain was identified as Enterobacter tabaci,and the fermentation broth significantly promoted the germination and seedling growth of tomato seeds.Fermentation broth at a concentration of 1×104cfu·mL-1had the best growth promoting effect.The root length,plant height,dry weight and fresh weight were higher by 63.42%,46.17%,150.00%and 144.83%,respectively,than in the control group.These results can provide technical support for the development of this strain as a microbial fertilizer or microbial plant growth promotion resource.

关 键 词：植物生长调节剂 分子鉴定 生物测定 肠杆菌 番茄 

分 类 号：S154.3[农业科学—土壤学]