梯度洗脱高效液相色谱法测定红花玉兰中4种植物激素  

Determination of 4 plant hormones in Magnolia wufengensis by gradient elution high performance liquid chromatography

作　　者：肖爱华 陈发菊 贾忠奎[1] 桑子阳 朱仲龙[1] 马履一[1,4] XIAO Aihua;CHEN Faju;JIA Zhongkui;SANG Ziyang;ZHU Zhonglong;MA Lüyi(Key Laboratory for Silviculture and Conservation of Ministry of Education,College of Forestry,Beijing Forestry University,Beijing 100083;Biotechnology Research Center,China Three Gorges University,Yichang 443002;Wufeng Institate of Forestry Science,Wufeng 443413;National Energy Research and Development Center for Non-food Biomass,Beijing Forestry University,Beijing 100083)

机构地区：[1]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083 [2]三峡大学生物技术研究中心,宜昌443002 [3]湖北省五峰土家族自治县科学研究所,五峰443413 [4]北京林业大学国家能源非粮生物质原料研发中心,北京100083

出　　处：《分析试验室》2020年第3期249-254,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(31570651);林业公益行业专项(201504704)项目资助。

摘　　要：建立了梯度洗脱高效液相色谱法测定红花玉兰中赤霉素(GA3)、生长素(IAA)、脱落酸(ABA)和玉米素(ZT)等4种植物激素的方法。采用Agilent ZORBAX SB-C18柱和紫外检测器,以甲醇和0.1 mol/L乙酸作为流动相进行梯度洗脱,流速1 mL/min,进样量10μL。GA3,IAA和ABA的检测波长为254 nm,柱温35℃;ZT的检测波长为270 nm,柱温40℃。采用外标法进行定量测定,4种植物激素的相关系数均大于0.9990。4种激素的回收率为98.1%~125.2%,相对标准偏差为0.31%~0.92%,日内和日间精密度RSD均<10%。方法可适用于红花玉兰多种组织的植物激素测定,为红花玉兰生长发育特性的研究奠定了基础。A method for the determination of four plant hormones,including gibberellin(GA3),auxin(IAA),abscisic acid(ABA)and zeatin(ZT)in Magnolia wufengensis,was established by gradient elution high performance liquid chromatography(HPLC)by using Agilent ZORBAX SB-C18 column and VWD.The mobile phase consisted of methanol and 0.1 mol/L acetic acid was used as gradient elution at a flow rate of 1 mL/min and an injection volume of 10μL.The detection wavelength of GA3,IAA and ABA was 254 nm,and the column temperature was 35℃.The detection wavelength of ZT was 270 nm,and the column temperature was 40℃.The external standard method was used for quantitative determination,and the correlation coefficient of four plant hormones was greater than 0.9990.The recoveries of the four hormones was between 98.1%and 125.2%,and the relative standard deviations were between 0.31% and 0.92%.The within-day and day to day deviations were both less than 10%.The method was applicable for the determination of plant hormones in various plant tissues of Magnolia wufengensis.It laid a foundation for the research on growth characteristics of Magnolia wufengensis.

关 键 词：梯度洗脱 高效液相色谱 红花玉兰 植物激素 

分 类 号：O657.72[理学—分析化学]