稳定同位素稀释液质串联法测定抗菌纺织品中3种含氯防霉抗菌  

Determination of 3 kinds of chlorine-containing antiseptic and antibacterial agent in antibacterial textile by stable isotope dilution-liquid chromatography-tandem mass spectrometry

作　　者：李志刚[1] 戴鸽 LI Zhigang;DAI Ge(Zhejiang Industry Polytechnic College,Shaoxing 312000;Ningbo Shenzhou Knitting Co.,Ltd.,Ningbo 315000)

机构地区：[1]浙江工业职业技术学院,绍兴312000 [2]宁波申洲针织有限公司,宁波315000

出　　处：《分析试验室》2020年第3期336-340,共5页Chinese Journal of Analysis Laboratory

摘　　要：建立了抗菌纺织品中三氯生、三氯卡班以及五氯酚等3种含氯抗菌剂的同位素稀释-液相色谱串联质谱的分析方法。样品用甲醇超声提取,聚酰胺粉净化。以水/乙腈为流动相,采用梯度洗脱,在Waters Acquity UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm)上进行分离,以多反应监测负离子模式进行检测,内标法定量。三氯生和五氯酚在0.5~100.0μg/L,三氯卡班在0.1~50.0μg/L范围内具有良好的线性,样品的回收率为80.0%~110.0%,相对标准偏差(RSD)为1.6%~5.4%,方法的定量限为0.1~0.5μg/kg,检出限为0.03~0.16μg/kg。方法前处理简单,试剂可用于纺织品中三氯生、三氯卡班和五氯酚的含量检测。A method was developed for the determination of chlorine-containing antiseptic and antibacterial agent including triclosan(TCS),triclocarban(TCC)and pentachlorophenol(PCP)in antibacterial textile by isotope dilution-liquid chromatography-tandem mass spectrometry.The sample was extracted by MeOH and purified by polyamide.The LC separation was performed by using a Waters Acquity UPLC BEH C18 column(2.1 mm×100 mm,1.7μm)with linear gradient elution program.Electrospray ionization was applied,and the detection was operated in the negative ion mode.TCS and PCP were linear in the range of 0.5-100.0μg/L.TCC was linear in the range of 0.1-50.0μg/L.The recoveries were 80.0%-110.0%,with the relative standard deviations(RSDs)of 1.6%-5.4%.The limits of quantitation(LOQs)and limits of detection(LODs)were 0.1-0.5μg/kg and 0.03-0.16μg/kg,respectively.This method can be applied for the determinations of TCS,TCC and PCP in textile.

关 键 词：同位素稀释 液相色谱质谱法 抗菌纺织品 含氯防霉抗菌剂 

分 类 号：O657[理学—分析化学]