氟苯尼考在海水鱼循环水养殖系统中的迁移转化规律  

Migration and Transformation of Florfenicol in Recirculating Aquaculture Systems

作　　者：郭睿 张莺脐 张羡宇 刘述颖 吴子曾 张倩 刘鹰[1,2] MD MOSTAFIZUR Rahman GUO Rui;ZHANG Yingqi;ZHANG Xianyu;LIU Shuying;WU Zizeng;ZHANG Qian;LIU Ying;MD MOSTAFIZUR Rahman(College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China;Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian 116023, China)

机构地区：[1]大连海洋大学海洋科技与环境学院,辽宁大连116023 [2]大连海洋大学,设施渔业教育部重点实验室,辽宁大连116023

出　　处：《水产科学》2020年第3期332-340,共9页Fisheries Science

基　　金：国家重点研发计划项目(2017YFB0404000);辽宁省海洋与渔业厅项目(201731);辽宁省高水平创新团队境外培养项目(201818);工业生态与环境工程教育部重点实验室开放课题(KLIEEE-17-12).

摘　　要：随着抗生素的大量使用,许多国家针对氟苯尼考残留制定了食品安全限量标准,并规定了相应的休药期。设计连续7 d以10 mg/(kg·d)的氟苯尼考投药试验和14 d停药的暴露试验,使用高效液相色谱检测氟苯尼考在循环水养殖系统中的残留规律以及在欧洲舌齿鲈肌肉和肝脏组织中的残留水平。试验结果显示,循环水养殖系统各处理单元中(蛋白分离器、生物滤池、紫外消毒装置)氟苯尼考的质量浓度随着投药时间的增加,呈不同程度积累上升。在投药第7 d时,各处理单元氟苯尼考的残留质量浓度均达到最大值;停药后,氟苯尼考残留质量浓度逐渐下降,停药第14 d各处理单元中的氟苯尼考水平与投药前水平相当。不同处理单元对于氟苯尼考降解率的贡献为紫外消毒装置>蛋白分离器>生物滤池。此外,氟苯尼考在欧洲舌齿鲈组织样品中的残留水平随停药时间延长而降低,停药7 d后其肝脏和肌肉组织中的残留量均低于国家标准(1000μg/kg)。本试验将为循环水系统中各水处理单元的工艺设计提供理论基础,推动水产养殖业的健康可持续发展。In many countries,food safety limit standards have been established for florfenicol residues in food and the corresponding drug withdrawal period has been specified with the extensive usage of antibiotics.In this study,exposure experiment was conducted for 7 days administration of florfenicol-containing diet at 10 mg/(kg·d)and 14 days discontinuation of the drug in recirculating aquaculture systems(RAS).HPLC was used to detect the residual florfenicol in RAS,muscle and liver tissues of toothed-tongue seabass Dicentrarchus labrax.The results showed that the concentration of florfenicol in the units of RAS(Protein skimmer,biofilter,and UV disinfection device)was increased with different degrees.The maximum value of florfenicol was observed at the 7th day of administration.Then the residual concentration of florfenicol was gradually decreased to the pre-treated level at the 14th day of drug withdrawal.At the same time,the contribution of florfenicol removal in different treatment units:UV disinfection device>protein skimmer>biofilter.Meanwhile,the residual level of florfenicol in fish tissues was decreased with the prolonged withdrawal time,and the residual florfenicol in muscle tissue was always higher than that in florfenicol in liver tissue.After 7 days withdrawal,the residual amount in liver and muscle tissues was lower than the national standard of 1000μg/kg.The findings will provide a theoretical basis for the process design of RAS,and contribute to the sustainable and healthy development of aquaculture industry.

关 键 词：欧洲舌齿鲈 氟苯尼考 循环水养殖系统 残留水平 高效液相色谱 

分 类 号：S969.39[农业科学—水产养殖]