快速老化小鼠红细胞和肌肉功能的增龄性变化及相关性研究  

Age-related changes and correlation of erythrocyte and muscle function in senescence-accelerated mouse

作　　者：王雅兰 吕欣 葛宝金 周东涛 周德正 黄国伟[1] 常红[1,2] WANG Yalan;LYU Xin;GE Baojin;ZHOU Dongtao;ZHOU Dezheng;HUANG Guowei;CHANG Hong(School of Public Health,Tianjin Medical University,Tianjin 300070,China;Faculty of Rehabilitation Medicine,Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津医科大学公共卫生学院,300070 [2]天津医科大学康复医学系,300070

出　　处：《重庆医学》2020年第9期1377-1380,1386,共5页Chongqing medicine

基　　金：国家自然科学基金项目(81730091,81472967)。

摘　　要：目的探讨快速老化小鼠红细胞和肌肉功能的增龄性变化,为衰老相关研究提供可靠适合的动物模型。方法选择4月龄和10月龄快速老化小鼠亚系8(SAMP8)及10月龄抗快速老化小鼠亚系1(SAMR1)小鼠各10只,采用老化度评分评估小鼠老化程度,以红细胞数目、血红蛋白(Hb)水平、红细胞分布宽度(RDW)变异系数(RDW-CV)及RDW标准差(RDW-SD)评估红细胞功能,相对肌肉质量、抓力、转棒式疲劳实验和游泳实验评估小鼠肌肉功能。结果老化度评分显示,10月龄SAMP8小鼠评分高于同月龄SAMR1小鼠,表现出更严重的老化状态(P<0.05);红细胞功能评估显示,10月龄SAMP8小鼠较4月龄SAMP8小鼠红细胞数目和Hb水平下降(P<0.05),RDW增加(P<0.05);肌肉功能评估显示,10月龄SAMP8小鼠肌肉质量明显低于同月龄SAMR1及4月龄SAMP8小鼠(P<0.05),肌肉力量和肌肉耐力下降(P<0.05),游泳速度减慢(P<0.05);相关性分析结果显示红细胞数目、Hb水平与抓力之间呈正相关(r=0.407、0.416,P<0.05)。结论SAMP8小鼠在衰老过程中表现出增龄性红细胞和肌肉功能的下降,可作为研究年龄相关造血和肌肉功能衰退的动物模型。Objective To study the age-related changes of erythrocyte and muscle function in senescence-accelerated mouse prone 8(SAMP8)to provide a reliable and suitable animal model for aging-related research.Methods Each 104-month-old and 10-month-old SAMP8 and 10-month-old senescence-accelerated mouse prone 1(SAMR1)were selected.The aging degree was evaluated by adopting the senescence scoring,the erythrocyte function was evaluated by the red blood cell(RBC)count,hemoglobin(Hb)level,RBC distribution width-coefficient of variation(RDW-CV)and RDW-standard deviation(RDW-SD),and the mouse muscle function was evaluated by the relative muscle mass,grip force,rotating-rod fatigue test and swimming test.Results The senescence scores indicated that the score in 10-month-old SAMP8 was higher than that in the same month old of SAMR1,showing more serious aging status(P<0.05);the erythrocyte function evaluation showed that compared with 4-month-old SAMP8,the RBC count and Hb level in 10-month-old SAMP8 was decreased(P<0.05),while RDW was increased(P<0.05).The muscle function evaluation showed that the muscle mass in10-month-old SAMP8 was significantly lower than that in the same month old SAMP1 and 4-month-old SAMP8(P<0.05),the muscle strength and endurance were decreased(P<0.05)and the swimming speed was slowed(P<0.05).The correlation analysis results showed that the RBC count and hemoglobin level were positively correlated with the grip force(r=0.407,0.416,P<0.05).Conclusion SAMP8 exhibits a decrease of age-related erythrocyte and muscle function,during the aging process,which can serve as an animal model for studying the aging-related functional decline of hematopoiesis and muscle.

关 键 词：快速老化小鼠 红细胞功能 肌肉功能 老化 

分 类 号：R332[医药卫生—人体生理学]