槲皮素对妊娠子宫动脉血管的舒张作用及机制研究  

Relaxant effect of quercetin on uterine arterial vessels and its mechanism

作　　者：李清华[1] 赵淑洋 尹陵轩 司延如 张翠娟[3] 潘智芳 杨潍潍 LI Qinghua;ZHAO Shuyang;YIN Lingxuan;SI Yanru;ZHANG Cuijuan;PAN Zhifang;YANG Weiwei(Public Health and Management Experimental Teaching Center,Weifang Medical College,Weifang,Shandong 261053,China;School of Biosciences and Technology,Weifang Medical College,Weifang,Shandong 261053,China;Department of Obstetrics,Affiliated Hospital of Weifang Medical College,Weifang,Shandong 261042,China;Shandong Provincial Key Laboratory of Biopharmacy,Weifang,Shandong 261053,China)

机构地区：[1]潍坊医学院公共卫生与管理实验教学中心,山东潍坊261053 [2]潍坊医学院生物科学与技术学院,山东潍坊261053 [3]潍坊医学院附属医院产科,山东潍坊261042 [4]山东省生物药物重点实验室,山东潍坊261053

出　　处：《重庆医学》2020年第9期1400-1404,共5页Chongqing medicine

基　　金：国家自然科学基金项目(81601318);山东省医药卫生科技发展计划项目(2016WS0668);2016年度山东省政府公派出国留学项目。

摘　　要：目的探讨槲皮素对妊娠子宫动脉内皮血管的舒张作用及其机制。方法利用妊娠大鼠子宫动脉收缩张力反应,观察槲皮素对大鼠内皮完整子宫动脉血管舒缩反应性的作用;使用槲皮素处理妊娠大鼠子宫动脉环后,采用Western blot法检测血管中磷酸化的内皮型一氧化氮合酶(eNOS)和总eNOS的表达水平;观察eNOS特异性抑制剂N-硝基-L-精氨酸甲酯(L-NAME)对槲皮素舒张大鼠血管功能的影响。结果槲皮素能剂量依赖性地舒张苯肾上腺素(PE)预收缩的妊娠大鼠子宫动脉血管,相比胸主动脉,子宫动脉对槲皮素的反应更加敏感,舒张幅度更大(P<0.01)。Western blot结果显示,经槲皮素处理子宫动脉后,大鼠血管内eNOS的磷酸化水平明显上升,并呈剂量依赖效应(P<0.01)。使用L-NAME预处理子宫动脉血管后,槲皮素对子宫动脉血管的舒张作用明显降低(P<0.01)。细胞实验发现,槲皮素在人内皮细胞系中也能明显增强eNOS的磷酸化水平和一氧化氮(NO)的产生(P<0.01)。结论槲皮素能剂量依赖性地舒张妊娠大鼠子宫动脉血管,可能与eNOS磷酸化水平升高有关。Objective To investigate the relaxant effect of quercetin on uterine arterial endothelial vessel and its mechanisms.Methods The effect of quercetin on the intact endothelial uterine artery vasoconstriction reactivity in rat was observed by using the contractile tension reaction of uterine artery in pregnant rats.After treating the rat uterine arterial ring by using quercetin,the Western blot method was adopted to detect the phosphorylated endothelial nitric oxide synthase(eNOS)in the blood vessel and total eNOS levels.The effect of eNOS specific inhibitor N-nitro-L-arginine methyl ester(L-NAME)on the function of quercetin for relaxing the rat blood vessels was observed.Results Quercetin could relax the phenylephrine(PE)precontracted uterine arterial vessel in pregnant rat with dose dependence.Compared with the thoracic aorta,the reaction of uterine artery on quercetin was more sensitive,and the diastole range was larger(P<0.01).The Western blot results showed that after treating the uterine artery by quercetin,the phosphorylation level of rat intravascular eNOS was significantly increased,moreover which showing the dose dependent effect(P<0.01).After pre-treating uterine arterial vessel by L-NAME,the relaxant effect of quercetin on uterine arterial vessel was significantly decreased(P<0.01).The cell experiment found that quercetin also could significantly enhance eNOS phosphorylation level in human endothelial cells and NO production(P<0.01).Conclusion Quercetin can relax the pregnant rat uterine arterial vessel in a dose-dependent manner,which may be related with the increase of eNOS phosphorylation level.

关 键 词：槲皮素 子宫动脉 血管舒张 内皮型一氧化氮合酶 子痫前期 

分 类 号：R714.2[医药卫生—妇产科学]