珍稀濒危植物独叶草SCoT-PCR反应体系的建立与优化  

Establishment and Optimization of SCoT-PCR Reaction System of Rare and Endangered Species Kingdonia uniflora Balf.F.et W.W.Smith

作　　者：张妙娟 张若晨 ZHANG Miaojuan;ZHANG Ruochen(Shanxi Yuncheng Vocational and Technical College of Agriculture,Yuncheng 044000,Shanxi,China)

机构地区：[1]山西运城农业职业技术学院,山西运城044000

出　　处：《种子》2020年第4期50-54,共5页Seed

基　　金：国家自然科学基金项目(30470133)。

摘　　要：采用正交设计法对影响珍稀濒危植物独叶草SCoT-PCR反应体系的主要因素进行优化,建立最佳的独叶草SCoT-PCR反应体系,并利用36份种质进行了验证。结果表明:在独叶草SCoT-PCR的最优反应体系(25μL)中:Mg^2+为1.5μL、dNTPs为1.50μL、Taq酶为0.3μL、Primer为1.2μL、DNA为3.0μL。建立的SCoT-PCR反应体系可用于该植物的遗传多样性和居群结构研究。In this paper,the main factors affecting start codon targeted polymorphism PCR(SCoT-PCR)for genetic diversity identification of rare endangered plant Kingdonia uniflora was optimized based on the orthogonal design method,and the optimal SCoT-PCR system was established and further verified by 36 germplasms.The results showed that the optimal reaction system of SCoT-PCR(25μL)was 1.5μL Mg^2+,1.5μL dNTPs,0.3μL Taq polymerase,1.2μL primers and 3.0μL DNA template.Finally,the SCoT-PCR system can be used to study the genetic diversity and population genetic structure of the plant.

关 键 词：珍稀濒危 独叶草 SCoT 反应体系 优化 

分 类 号：Q943[生物学—植物学]