Who is the“murderer”of the bloom in coastal waters of Fujian,China,in 2019?  

作　　者：CEN Jingyi WANG Jianyan HUANG Lifen DING Guangmao QI Yuzao CAO Rongbo CUI Lei Lü Songhui 

机构地区：[1]College of Life Science and Technology,Jinan University,Guangzhou 510632,China [2]Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Blooms of Guangdong Higher Education Institutes,Guangzhou 510632,China [3]Department of Science Research,Beijing Museum of Natural History,Beijing 100050,China [4]Fujian Fishery Resources Monitoring Center,Fuzhou 350003,China

出　　处：《Journal of Oceanology and Limnology》2020年第3期722-732,共11页海洋湖沼学报（英文）

基　　金：Supported by the National Key R&D Program of China(No.2017YFC1404301);the National Natural Science Foundation of China(No.41606175);the Beijing Natural Science Foundation(No.8194059)。

摘　　要：On May 24-29,2019,a bloom occurring in Pingtan coastal areas of Fujian Province caused mass mortality of cage-cultured fish(Plectorhinchus cinctus and Pagrosomus major).During the bloom,two major causative organisms were present:Prorocentrum donghaiense(at a concentration of 1.46×10^7 cells/L)and an unknown naked dinoflagellate(4.58×10^6 cells/L).The naked dinoflagellate was isolated and cultured in this study,and its morphological features were examined using light microscopy and scanning electron microscopy.The large subunit(LSU)of the rRNA gene and the internal transcribed spacer(ITS)region of the naked dinoflagellate were also sequenced for field bloom samples and lab culture strains(PT-A and PT-B).On the basis of its morphological characteristics and molecular sequences,the unknown naked dinoflagellate was identified as Karlodinium digitatum.According to the phylogenetic analysis,the Karl.digitatum was most closely related to Karlodinium australe and Karlodinium armiger,and the three species clustered into a single clade of Karlodinium with bootstrap/posterior probability values of 95%/0.99 and 86%/0.99 inferred from LSU and ITS sequences,respectively.Karl.digitatum was first reported as Karenia digitata,a new harmful algal species bloomed in Hong Kong,China,in 1998.In present study,we gave a detailed morphological and phylogenetic description of Karl.digitatum and submitted the molecular sequences of this species to GenBank for the first time.

关 键 词：KARENIA digitata KARLODINIUM digitatum harmful ALGAL BLOOM Ichthyotoxicity 

分 类 号：X55[环境科学与工程—环境工程]