bHLH genes polymorphisms and their association with growth traits in the Pacifi c oyster Crassostrea gigas  

作　　者：CHEN Na LI Li LI Chenghua LIN Zhihua MENG Jie LIU Sheng SONG Kai BAO Yongbo 

机构地区：[1]Zhejiang Key Laboratory of Aquatic Germplasm Resources,College of Biological&Environmental Sciences,Zhejiang Wanli University,Ningbo 315121,China [2]School of Marine Sciences,Ningbo University,Ningbo 315211,China [3]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China

出　　处：《Journal of Oceanology and Limnology》2020年第3期862-868,共7页海洋湖沼学报（英文）

基　　金：Supported by the National Key R&D Program of China(No.2018YFD0901400);National Natural Science Foundation of China(No.31672678);Ningbo Municipal Science and Technology International Cooperation Research Projects(No.2016D10017);the Zhejiang Provincial Major Program of Science and Technology(No.2016C02055-9)。

摘　　要：The basic helix-loop-helix(bHLH)genes encode a large superfamily of transcription factors in the Pacific oyster(Crassostrea gigas),and play a very important role in regulation of growth and development.To investigate the oyster growth traits and the associations with bHLH genes variations,we analyzed the gene polymorphisms-traits association in a wild population,and confirmed the results in another independent wild population by targeted gene re-sequencing and SNPshot analysis.After screening the single nucleotide polymorphisms(SNPs)in three candidate genes of the bHLH family(88 bHLH genes in two wild oyster populations in total),we identified the allele CgLoblHLH4-T/G located in the exon of the CgLoblHLH4 gene.This allele is a non-synonymous mutation(Phe/Leu)with an extremely significant association with shell width(P<0.01)and allele G is beneficial to shell width.This SNP on the CgLoblHLH4 gene might have a potential value as a genetic marker of growth traits that could be used in breeding in C.gigas in the future.

关 键 词：CRASSOSTREA GIGAS basic helix-loop-helix(bHLH) RE-SEQUENCING single nucleotide polymorphism(SNP)shot growth traits 

分 类 号：Q953[生物学—动物学]