Comparative analysis of the ecological fitness and transcriptome between two genotypes of the brown planthopper Nilaparvata lugens  

作　　者：LIU Kai CHEN Zhan SU Qin YUE Lei CHEN Wei-wen ZHANG Wen-qing 

机构地区：[1]State Key Laboratory of Biocontrol/School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第6期1501-1511,共11页农业科学学报（英文版）

基　　金：This work was funded by the National Natural Science Foundation of China(31672021).

摘　　要：Insect quantity is an important index for determining the degree of damage to plants.The brown planthopper(BPH),Nilaparvata lugens(Stal),is one of the most important rice pests in Asia.Here,we used two genotypes of BPH,a highfecundity genotype(HFG)and a low-fecundity genotype(LFG),to measure their ecological fitness and to explore possible causes for genotype differentiation by transcriptome sequencing.Our results showed that there were significant differences in body weight,egg hatching rate and honeydew secretion between the HFG and LFG,particularly,the number of eggs laid per female was more significant.Transcriptional analysis showed that a total of 1966 differentially expressed genes(DEGs)were mainly involved in energy metabolism,carbohydrate and lipid syn thesis,in sect horm one syn thesis and fecun dity-related pathways.Moreover,we found that the phosphorylation levels of AMPK,AKT and FoxO and the synthesis of ecdysone were different between the two genotypes of BPH.These findings may partially explain the differenee in the ecological fitness between HFG and LFG.This study is the first to compare the differences between two genotypes of BPH and to provide transcriptomic evidenee to further understand the fecundity of BPH.

关 键 词：NILAPARVATA LUGENS genotype FECUNDITY RNA-seq phosphorylation 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]