何首乌饮对衰老大鼠肝脏胰岛素信号通路的影响  

Effect of Heshouwuyin on Liver Insulin Pathway of Aging Rats

作　　者：王梦琦 杨宏曦 康硕 牛嗣云 WANG Mengqi;YANG Hongxi;KANG Shuo;NIU Siyun(Medical College,Hebei University,Baoding Hebei,071000)

机构地区：[1]河北大学医学院,河北保定071000

出　　处：《贵州中医药大学学报》2020年第1期17-20,32,共5页Journal of Guizhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目:基于表观遗传学理论研究补肾中药何首乌饮对肾精亏虚雄性大鼠生殖衰老调控的甲基化机制项目,项目编号:81673714。

摘　　要：目的:探讨何首乌饮对衰老大鼠肝脏胰岛素信号通路的关键基因IR、IRS-1表达的影响。方法:选用9月龄雄性大鼠分为4组即正常对照组,衰老模型组,何首乌饮组,自然衰老组。饲养到16月龄时取材,qRT-PCR检测肝脏组织IRS1、IRS基因表达的变化;HE染色观察肝脏组织细胞的形态学改变。结果:何首乌饮组和正常对照组的IR、IRS1的基因表达水平明显高于自然衰老组和衰老模型组(P<0.01)。HE染色可见衰老模型组、自然衰老组显示衰老征象,何首乌饮组较衰老模型组、自然衰老组衰老征象表现相对较轻。结论:调控胰岛素信号通路中关键基因的表达水平,达到减缓大鼠肝脏组织衰老的作用。Objective:To observe the effect of Heshouwuyin on the expression of insulin pathway IR and IRS-1 genes in liver tissue of aging rats.Methods:Sixty male rats of clean grade 9 months were selected to divide into 4 groups:normal control group,aging model group,natural aging group,and Heshouwuyin group.After feeding 16 months,all groups were taken materials and reserved.Real-time fluorescence quantitative PCR was used to detect the expression of IRS1 and IR genes in liver tissues,HE staining were used to observe the aging of liver tissue.Results:The expression of IR and IRS1 in the group of Heshouwuyin and control group were significantly higher than that of the natural aging group and the model group(P<0.01).Through HE staining,the natural aging group and model group showed aging signs,the Heshouwuyin and young group’s aging signs in relatively lower level than the model group.Conclusions:Heshouwuyin could delay the aging of rat liver by increasing the expression of IRS1 and IR,the key gene of insulin pathway in the liver of aging rats.

关 键 词：何首乌饮 衰老 胰岛素信号通路 肝脏 

分 类 号：R285.5[医药卫生—中药学]