油菜黑胫病菌(Leptosphaeria biglobosa)T-DNA插入突变体表型特征分析  

Analysis of phenotypic characteristics of T-DNA insertion mutants in Leptosphaeria biglobosa

作　　者：史志丹 宋培玲 郝丽芬 皇甫海燕 燕孟娇 杨永青 吴晶 赵丽丽 李子钦[1,2] SHI Zhidan;SONG Peiling;HAO Lifen;HUANGFU Haiyan;YAN Mengjiao;YANG Yongqing;WU Jing;ZHAO Lili;LI Ziqin(College of Life Sciences,Inner Mongolia University,Hohhot 010070,China;Plant Protection Institute,Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古大学生命科学学院,内蒙古呼和浩特010070 [2]内蒙古自治区农牧业科学院植物保护研究所,内蒙古呼和浩特010031

出　　处：《北方农业学报》2020年第1期75-80,共6页Journal of Northern Agriculture

基　　金：内蒙古农牧业科技创新基金项目(2017CXJJN15);内蒙古农牧业青年创新基金项目(2018QNJJN06,2017QNJJN04)。

摘　　要：【目的】鉴定油菜黑胫病菌T-DNA随机插入突变体的表型特征。【方法】通过农杆菌介导的遗传转化体系获得黑胫病菌突变体,对这些突变体的生物学特性、致病性、胞外酶进行研究。【结果】大部分突变体的菌落形态与野生型菌株无明显差异;突变体生长速度和产孢量均低于野生型菌株,只有1个突变体产孢量显著高于野生型菌株;所测突变体中53%的突变体与野生型菌株相比,致病性无显著差异;有7个突变体致病性显著升高,突变体和野生型菌株均可分泌蛋白酶和淀粉酶,不分泌果胶酶和纤维素酶。【结论】获得了与野生型菌株表型最为相近的突变体T34,为黑胫病菌与寄主互作机制及其致病机制研究奠定了基础。【Objective】To identify the phenotypic characteristics of random T-DNA insertion mutants in Leptosphaeria biglobosa of oilseed rape.【Methods】L.biglobosa mutants were obtained by Agrobacterium-mediated genetic transformation system,and the L.biological characteristics,pathogenicity,and extracellular enzymes of these mutants were studied.【Results】The colony morphology of most mutants was not significantly different from that of wild-type strain.The growth rate and sporulation yield of the mutant were lower than that of the wild-type strain,and the sporulation yield of only one mutant was significantly higher than that of the wild-type strain.Compared with the wild-type strain,53%of the mutants had no significant difference in pathogenicity.The pathogenicity of 7 mutants was significantly increased.Both mutants and wild-type strains could secrete protease and amylase,but not pectinase and cellulase.【Conclusion】The mutant T34 with the most similar phenotype to the wild-type strain was obtained,which laid a foundation for the study of the interaction mechanism between L.biglobosa and its host and its pathogenic mechanism.

关 键 词：油菜 LEPTOSPHAERIA biglobosa 突变体 表型 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]