丝素蛋白/多巴胺医用粘合剂的制备及其性能  

Preparation and properties of silk fibroin/dopamine medical adhesive

作　　者：刘涵 尹子楚 蔡玉荣[1] 姚菊明[1] LIU Han;YIN Zichu;CAI Yurong;YAO Juming(School of Materials Science and Engineering,Zhejiang Sci-Tech University,Hangzhou 310018,China)

机构地区：[1]浙江理工大学材料科学与工程学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2020年第3期308-314,共7页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家自然科学基金项目(51672251);浙江省新苗人才计划(2019R406060)。

摘　　要：为了获得兼具良好生物相容性及较强粘合强度的生物医用粘合剂,以丝素蛋白为基质和NHS/EDC为交联剂,在丝素蛋白上接枝多巴胺;采用京尼平对丝蛋白-多巴胺材料进行共价交联,从而制得了丝素蛋白/多巴胺医用粘合剂;通过正交实验的方法,分析多因素对粘合剂粘合强度的影响,优化制备工艺。结果表明:丝素蛋白浓度、反应时间、NHS/EDC的使用量、多巴胺的添加量以及京尼平的用量均可影响所得粘合剂的粘合强度。当丝素蛋白浓度为12%、多巴胺与丝素蛋白羧基摩尔比为1∶6,以及京尼平与丝素蛋白氨基摩尔比为2∶1时,所制得的粘合剂性能最佳;优化条件下制得的粘合剂在37℃条件下具有最高的粘合强度,且此强度随使用时间的增长而增大,12 h时可达到(181.49±42.82) kPa,是临床上正在使用的纤维蛋白胶的粘合强度的18倍。制备的粘合剂具有较好的可降解性,在临床上具有一定的应用前景。In order to obtain biomedical adhesives with high biocompatibility and strong adhesive properties, the silk fibroin was used as a matrix and NHS/EDC was used as a cross-linking agent to graft dopamine on the silk fibroin. Then, genipin was applied for covalent cross-linking of silk fibroin-dopamine material to prepare silk fibroin/dopamine medical adhesive. The influence of multiple factors on the bond strength of the adhesive was explored and the preparation process was optimized by the orthogonal experiment. The results show that silk fibroin concentration, reaction time, dose of NHS/EDC, addition volume of dopamine and dose of genipin can influence the bond strength of the adhesive. The adhesive prepared can reach the optimal performance under the following conditions: The concentration of silk fibroin 12%, the molar ratio of grafted dopamine to silk fibroin 1∶6 and the molar ratio of genipin to amino group 2∶1. The adhesive has the highest bond strength at 37 ℃ under the optimized conditions, and such strength increases with the use time and can reach(181.49±42.82) kPa at 12 h, 18 times higher than the bond strength of fibrin glue used clinically. The adhesive prepared has good biodegradability and owns certain clinical application prospect. During the lap shear test, the tensile strength of optimized adhesive increased with time and reached(181.49±42.82) kPa at 12 h under 37 ℃, which is 18 times higher than fibrin glue for clinical use. Therefore, this bioadhesive has a good application prospect in clinical practice.

关 键 词：丝素蛋白 多巴胺 京尼平 生物粘合剂 共价交联 

分 类 号：TB34[一般工业技术—材料科学与工程]