应用微卫星技术分析评价虎皮猫群体的遗传质量  

Evaluation on the Genetic Quality of Tiger Skin Population by Microsatellites

作　　者：王洪[1] 付瑞[1] 魏杰[1] 贾松华 光娇娜 马丽颖[1] 岳秉飞[1] WANG Hong;FU Rui;WEI Jie;JIA Songhua;GUANG Jiaona;Ma Liying;YUE Bingfei(National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院,北京102629

出　　处：《实验动物科学》2020年第1期1-6,共6页Laboratory Animal Science

基　　金：国家重大专项(No.2017ZX10304402002006)。

摘　　要：目的应用微卫星DNA标记检测法评价虎皮猫群体的遗传结构。方法应用37个微卫星位点对25只虎皮猫的DNA样本进行PCR扩增,利用软件POPGEN1.32,对扩增产物的测序结果进行统计分析,获得虎皮猫群体的遗传结构参数。结果该虎皮猫群体的平均观测等位基因数是4.7027,平均有效等位基因数是2.6907,平均有效杂合度是0.6021,平均多态性信息含量是0.5520。结论微卫星DNA标记检测法能够应用于虎皮猫群体的遗传质量检测,该虎皮猫群体的遗传结构符合封闭群实验动物的结构特征。Objective To evaluate the genetic structure of Tiger skin population by microsatellites.Method 37 microsatellites were applied to amplify the 25 genomic DNA by PCR.Software POPGEN1.32 was used to analyze the scanning result of PCR products.The parameters of genetic structure of Tiger skin population were obtained.Result The observed average number of alleles is 4.7027.The average effective average number of alleles is 2.6907.The average effective heterozygosity is 0.6021.The average polymorphism information content(PIC)is0.5520.Conclusion Microsatellites can be used to genetic quality monitoring of Tiger skin population.The genetic background of Tiger skin population is in conformity with the characters of closed colony.

关 键 词：实验用猫 微卫星 封闭群 遗传质量 

分 类 号：Q789[生物学—分子生物学]