七彩山鸡F亚群禽白血病病毒的分离与鉴定  

Isolation and Identification of Avian Leukosis Virus Subgroup F from Colorful Pheasant

作　　者：严立福 黎丽珍 郑晓翠 陈建 冯开荣 曹伟胜[1] YAN Lifu;LI Lizheng;ZHENG Xiaocui;CHEN Jian;FENG Kairong;CAO Weisheng(Key Laboratory of Zoonosis Prevention and Control of Guangdong Province,Guangdong Laboratory for Lingnan Modern Agriculture Science and Technology,Key Laboratory of Veterinary Vaccine Innovation Development,Ministry of Agriculture and Rural Affairs,College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong 510642;Jiangmen Animal Disease Prevention and Control Center,Jiangmen,Guangdong 529000)

机构地区：[1]华南农业大学兽医学院,农业农村部兽用疫苗创制重点实验室,岭南现代农业科学与技术广东省实验室,广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642 [2]江门市动物疫病预防控制中心,广东江门529000

出　　处：《中国家禽》2020年第4期41-45,共5页China Poultry

基　　金：国家重点、研发计划项目(2016YFD0501606);广东省家禽产业技术体系疾病控制岗位专家(2019KJ128);现代农业产业技术体系建设专项资金(CARS-41-G16)。

摘　　要：为了解广东某七彩山鸡种禽场禽白血病流行情况,通过接种DF-1细胞、ELISA抗原检测、聚合酶链式反应(PCR)、囊膜基因克隆测序等方法分离并鉴定出两株ALV-F,分别命名为FGD1801和FGD1802。为进一步了解分离株遗传进化特点,利用PCR方法扩增出env基因并测序,同时与各亚群参考毒株的gp85核苷酸序列进行对比。结果显示:这两个分离株env基因gp85片段长度都为1080 bp,预计编码360个氨基酸,FGD1801和FGD1802分离株gp85基因核苷酸序列的相似性为92.8%,与A、B、E、J和K亚群共26株ALV参考毒株相似性在47.0%~64.5%之间,而与F亚群参考株的相似性在92.0%~92.5%,显著高于其他亚群,且位于同一进化分支上。研究表明,从七彩山鸡分离的FGD1801和FGD1802属于ALV-F,是我国华南地区新发现的ALV亚群。To investigate the prevalence of colorful pheasant breeder farm infected by avian leukosis virus in Guangdong province of China,two strains of ALV-F designed as FGD1801 and FGD1802 were isolated and identified by inoculating plasma into DF-1 cells,antigen detection by ELISA,polymerase chain reaction(PCR),and membrane gene cloning and sequencing.In order to further understand the genetic evolution characteristics of isolates,the env genes of ALV-F isolates were compared with reference strains of other subgroup ALV.The results showed that the gp85 fragments of the two ALV-F isolates were 1080 bp in length and were expected to encode 360 amino acids.The nucleotide sequence similarity of gp85 among FGD1801,FGD1802 and the ALV-F reference strain was 92.0%to92.8%,while the similarity of gp85 gene with those of 26 reference strains in subgroups A,B,E,J and K were ranged from 47.0%to 64.5%.All the ALV-F isolates were located in the same evolutionary branch,greatly different from other ALV subgroups.The results indicated that the strains FGD1801 and FGD1802 isolated from colorful pheasants should belong to ALV-F,which was a newly discovered ALV subgroup in Southern China.

关 键 词：七彩山鸡 F亚群禽白血病病毒 GP85基因 

分 类 号：S855.3[农业科学—临床兽医学]