硫化氢对低温胁迫下甜樱桃柱头和子房线粒体功能的影响  

Effects of hydrogen sulfide on mitochondrial function in sweet cherry stigma and ovary under low temperature stress

作　　者：魏国芹[1] 陶吉寒[1] 付全娟 侯森[1] 杨兴华[1] 隋曙光[1] 余贤美[1] 孙玉刚[1] WEI Guo qin;TAO Ji han;FU Quan juan;HOU Sen;YANG Xing hua;SUI Shu guang;YU Xian mei;SUN Yu gang(Shandong Institute of Pomology,Tai’an 271000,Shandong,China)

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《应用生态学报》2020年第4期1121-1129,共9页Chinese Journal of Applied Ecology

基　　金：山东省农业科学院创新工程项目、山东省果树研究所青年科研基金项目(2018KY03);中波农业科技中心项目资助。

摘　　要：为探索低温胁迫下外源硫化氢(H2S)对甜樱桃花的柱头和子房线粒体功能的影响,本研究以甜樱桃品种‘早大果’花枝为试材,在-2℃低温下喷施0.05 mmol·L^-1硫氢化钠(NaHS,H2S供体)和15μmmol·L^-1次牛磺酸(HT、H2S清除剂),测定柱头和子房线粒体中活性氧、抗氧化酶和线粒体膜通透性转换孔(MPTP)开放程度、膜流动性、膜电位和细胞色素(Cyt c/a)比值变化。结果表明:低温胁迫导致线粒体内过氧化氢(H2O2)和丙二醛(MDA)含量显著增加,线粒体MPTP明显增大,膜流动性降低,膜电位和线粒体Cyt c/a吸光度比值、膜H+-ATPase活性显著下降,线粒体结构受到损伤。低温胁迫下,外施0.05 mmol·L^-1 NaHS可显著降低低温胁迫下柱头和子房线粒体H2O2和MDA含量,在较长时间内维持较高的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,减小线粒体MPTP开放程度,增强线粒体膜流动性,提高线粒体膜电位、Cyt c/a值和膜H+-ATPase活性;NaHS清除剂HT则抵消NaHS对上述参数的影响。综上所述,外源H2S可以提高低温胁迫下甜樱桃柱头和子房线粒体抗氧化酶活性,减少H2O2和MDA积累,提高膜H+-ATPase活性,稳定线粒体膜结构和功能,进而缓解低温胁迫对花器官的伤害。To investigate the effects of H2S on mitochondrial functions under low temperature stress,we analyzed the effects of 0.05 mmol·L^-1 NaHS and 15μmmol·L^-1 HT(hypotaurine and H2S scavenger)on mitochondria antioxidant enzyme activities and mitochondrial permeability transition pore,mitochondrial membrane fluidity,mitochondrial membrane potential,Cyt c/a ratio and H+-ATPase activity in sweet cherry stigma and ovary with sweet cherry variety Zaodaguo under-2℃low temperature stress.The results showed that low temperature stress increased the concentrations of mitochondrial H2O2 and MDA,enhanced the mitochondrial membrane permeability,but decreased the mitochondrial membrane fluidity,membrane potential,Cyt c/a and H+-ATPase acti-vity.Application of NaHS at 0.05 mmol·L^-1 could effectively reduce the concentrations of H2O2 and MDA,and keep higher activities of SOD,POD and CAT of mitochondrial for longer time.Furthermore,application of 0.05 mmol·L^-1 NaHS could decrease mitochondrial membrane permeability while increase mitochondrial membrane fluidity,membrane potential,Cyt c/a and H+-ATPase activity in stigma and ovary under low temperature stress.The effects of NaHS were completely offset by HT addition.The results suggested that exogenous H2S could alleviate the oxidative damage on stigma and ovary stress through decreasing H2O2 accumulation,regulating mitochondria antioxidant system,increasing H+-ATPase activity,and mitigating mitochondria function under low temperature.

关 键 词：硫化氢 甜樱桃 低温胁迫 花 线粒体 

分 类 号：S662.5[农业科学—果树学]