不同化学型互叶白千层优良单株选择及种质资源分子鉴别  

Selection of Elite Individual Plants of Melaleuca alternifolia with Different Chemical Components and Molecular Identification of Germplasm Resources of M.alternifolia

作　　者：覃子海[1] 唐庆兰[1] 梁忠云[1] 肖玉菲[1] 张烨[1] 张晓宁[1] 刘海龙[1] QIN Zihai;TANG Qinglan;LIANG Zhongyun;XIAO Yufei;ZHANG Ye;ZHANG Xiaoning;LIU Hailong(Guangxi Zhuang Autonomous Region Academy of Forestry/State Bureau of China Key Laboratory for Cultivation of Fast-growing Timber Trees in the Central South of China/Guangxi Key Laboratory for Cultivation of Superior Timber Trees,Nanning,Guangxi 530002)

机构地区：[1]广西壮族自治区林业科学研究院/国家林业局中南速生材繁育实验室/广西优良用材林资源培育重点实验室,广西南宁530002

出　　处：《热带农业科学》2020年第2期39-45,共7页Chinese Journal of Tropical Agriculture

基　　金：广西科技计划项目(No.桂科AD18281083)。

摘　　要：通过初选、复选、决选获得4-松油醇型互叶白千层优良单株7株,单株得油量均大于10 g(11.30~26.58 g),含油率为1.00%~1.46%,精油中4-松油醇含量为38.04%~40.27%,1,8-桉叶素含量为1.09%~2.21%;获得1,8-桉叶素行互叶白千层优良单株22株,单株含油率>1.5%(1.521%~3.329%),精油的特征组分为1,8-桉叶素含量>60%(63.04%~69.61%),4-松油醇含量>1.0%(1.051%~2.528%),α–蒎烯:1%~5%,苧烯:5%~10%,α–松油醇:6%~15%。同时,本研究选取28份不同化学型的互叶白千层材料,采用SSR引物进行引物筛选和分子鉴别,获得多态性谱带64条,多态率为94.12%。遗传一致度为0.3035~0.8971;遗传距离为1.0075~9.4128。其中SSR标记将14个桉叶素型与9个4–松油醇型区分开。本研究结果为互叶白千层种质资源分子鉴别奠定了研究基础。Seven elite individual trees of Melaleuca alternifolia containing high 4-terpineol were selected through primary selection, selection and final selection. These individual trees produced essential oil more than 10 g(11.30 g-26.58 g)/tree, and contained 1.00%-1.46% of essential oil, and the essential oil contained 38.04%-40.27% of 4-terpineol and 1.09%-2.21% of 8-cineole. Twenty two elite individuals of M.alternifolia containing 1, 8-cineole were selected and their essential oil content was more than 1.5%(1.521%-3.329%)/tree;the characteristic components of the essential oil of these individuals included 1,8-cineole(> 60%(63.04%-69.61%)), 4-terpineol(> 1.0%(1.051%-2.528%)), α – pinene(1%-5%), limonene(5%-10%), and α – terpinene( 6%-15%). At the same time 28 accessions of M. alternifolia with different chemical components were selected for molecular identification by using SSR primers, and 64 polymorphism bands were regenerated with a polymorphism rate of 94.12%. The range of genetic consistency was 0.3035-0.8971, and the range of genetic distance was 1.0075-9.4128. Among the 28 accessions 14 accessions containing 1, 8-cineole and 9 accesions containing 4-terpineol were identified by using the SSR markers. This may lay a foundation for molecular identification of M. alternifolia germplasm resources.

关 键 词：互叶白千层 优良单株 4-松油醇 1 8-桉叶素 分子标记 

分 类 号：S722.3[农业科学—林木遗传育种]