miR-21在狼疮性肾病患者中的差异表达研究  

Expression of microRNA-21 in lupus nephritis and its clinical value

作　　者：罗秀霞 范世珍[2] 尹志华 戴莉萍 邓燕群 郑生康 李林煜龙 Luo Xiuxia;Fan Shizhen;Yin Zhihua;Dai Liping;Deng Yanqun;Zheng Shengkang;Lilin Yulong(Rheumatic Laboratory,Shenzhen Futian Hospital for Rheumatic Diseases,Shenzhen 518040,China;Shenzhen Hospital,Guangzhou University of Chinese Medicine,Shenzhen 518034,China;Peking University Shenzhen Hospital,Shenzhen 518036,China)

机构地区：[1]深圳市福田区风湿病专科医院风湿病实验室,518040 [2]广州中医药大学深圳医院,518034 [3]北京大学深圳医院,518036

出　　处：《国际医药卫生导报》2020年第9期1194-1197,共4页International Medicine and Health Guidance News

基　　金：广东省医学科研基金项目(A2018089);深圳市福田区卫生公益性科研项目(FTWS2017020,FTWS2018005)。

摘　　要：目的本文主要探究miR-21在狼疮性肾病(LN)患者中的表达及意义。方法本文收集于2017年11月至2018年11月住院的30例已确诊的狼疮性肾病患者(女29例,男1例)及20例已确诊的系统性红斑狼疮(SLE)患者(女19例,男1例)标本(无狼疮性肾病)作为病例组标本,另外选择同期同年龄段的20例健康体检者(女19例,男1例)作为对照组。采用qRT-PCR检测技术对所有标本血清中miR-21的表达量进行检测,并利用SPSS软件进行统计学分析,比较病例组与对照组miR-21的表达水平与患者临床特征的关系。结果LN组血清中miR-21的水平明显高于对照组及SLE组[(1.46±0.21)比(0.84±0.46)、(1.18±0.07)],差异均有统计学意义(均P<0.05)。与对照组相比,LN组与SLE组标本的miR-21的表达水平、血清钙磷乘积、血清磷酸盐和血清hs-CRP均显著增加(均P<0.05),血清补体C3和C4均显著降低(均P<0.05)。LN组的miR-21、尿蛋白显著高于SLE组,血清钙、血清补体C3显著低于SLE组。miR-21诊断灵敏度为73.3%,特异性为93.0%,诊断特异性较其他指标显著增加。结论血清中miR-21今后可作为LN区别于非肾病性SLE诊断的参考指标。Objective To investigate the expression of microRNA-21 in the serum of lupus nephritis(LN)patients and its clinical value.Methods From November 2017 to November 2018,30 serum samples were collected from the LN patients(29 female cases,1 male case),20 serum samples were collected from the SLE patients(no LN)(19 female cases,1 male case),and 20 serum samples were collected from the healthy control(19 female cases,1 male case).The expression level of miR-21 was detected by qRT-PCR and statistically analyzed by SPSS.The relationship between the expression of miR-21 and clinical features were analyzed.Results The expression levels of miR-21 of LN group was significantly higher than those of the control group and SLE group[(1.46±0.21),(0.84±0.46),(1.18±0.07)](all P<0.05).Compared with the control group,the expression level of miR-21,serum product of calcium-phosphorus,serum phosphate,and serum hs-CRP of LN group and SLE group significantly increased,serum complement C3 and C4 levels significantly decreased(P<0.05).The expression levels of miR-21 and urine protein of LN group were significantly higher than those of SLE group,serum calcium and complement C3 levels were significantly lower than those of SLE group.The diagnostic sensitivity of miR-21 was 73.3%,the specificity was 93.0%.Conclusion The expression level of miR-21 in the serum can serve as a potential diagnostic marker of LN to distinguish from the no nephritis SLE in the future.

关 键 词：狼疮性肾病 系统性红斑狼疮 MIR-21 

分 类 号：R593.242[医药卫生—内科学]