顶空液相微萃取-高效液相色谱法测定水中六氯苯  

Determination of hexachlorobenzene in water by head-space liquid-phase microextraction high performance liquid chromatography

作　　者：周建刚[1] 陈红兵[1] ZHOU Jiangang;CHEN Hongbing(Faculty of Resources and Environmental Science, Hubei University, Wuhan 430062, China)

机构地区：[1]湖北大学资源环境学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2020年第3期267-271,共5页Journal of Hubei University：Natural Science

基　　金：湖北省教育厅教学研究项目(2018221);湖北省高等学校实验室研究项目(HBSY2018-05);区域开发与环境响应湖北省重点实验室开放基金(2017(C)001)资助。

摘　　要：应用顶空液相微萃取技术建立水体中六氯苯的高效液相色谱分析方法.研究不同的萃取条件(萃取剂、液滴体积、萃取时间、搅拌速度、温度等)及测定条件对检测六氯苯的影响,确定最佳萃取条件:正辛醇作萃取剂,液滴体积10μL,搅拌速度550 r/min,60℃条件下萃取30 min.应用此方法测定自来水和湖水样中的六氯苯,相对标准偏差(RSD)分别为6.3%和6.85%(n=6),回收率分别为105.4%和107.1%.Head-space liquid-phase microextraction high performance liquid chromatography was established for the determination of hexachlorobenzene in water.In order to optimize the experimental conditions,the effects of different extraction(extraction solvent,drop volume,extraction time,stirring rates and temperature)and measuring conditions on detection of hexachlorobenzene were investigated.Optimal experimental conditions were as follows:organic solvent,drop volume,stirring rate,solution temperature,and extraction time were:octanol,10μL,550 r/min,65℃and 30 min,respectively.The method was used to determine hexachlorobenzene residues in the tap water and lake,the relative standard deviations of the method were 6.3%and 6.85%(n=6),and the recoveries were 105.4%and 107.1%,respectively.

关 键 词：顶空液相微萃取 六氯苯 高效液相色谱 

分 类 号：O6-3[理学—化学]