Spectral Characteristics and Quantitative Analysis of Troxerutin-Cu^2+ Complex  

作　　者：Yu LIN Liujun LIANG Wen LIU Zhenzhen PAN Fengfeng XIE 

机构地区：[1]Faculty of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530001, China

出　　处：《Asian Agricultural Research》2020年第3期60-63,共4页亚洲农业研究（英文）

基　　金：Basic Ability Enhancement Project for Young and Middle-aged Teachers in Colleges and Universities of Guangxi in 2017(2017KY0284);Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine(GXZYKF2019-7);Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine[(2013)No.20].

摘　　要：[Objectives]This study aims to establish a simple,convenient,fast,low-cost,low-consumption and efficient method for quality control of troxerutin.[Methods]In NaAc-phosphate buffer at pH=8,troxerutin could react with Cu^2+to form complex,which had a characteristic absorption peak at 408 nm in the ultraviolet spectrum.The content of troxerutin could be determined indirectly by measuring the absorbance of the complex.[Results]Within the linear range of 27.82-74.19μg/mL,the absorbance of the complex has a good linear relationship with the concentration of troxerutin.The regression equation is A=0.0119C-0.1384(r=0.9991),and the detection limit is 1.59μg/mL.This method used to determine the content of troxerutin in samples from different manufacturers,and the recovery is 100.9%-102.7%.[Conclusions]The ultraviolet spectrophotometry is used in this experiment to indirectly determine the content of troxerutin by measuring the absorbance of troxerutin-Cu^2+complex,and the results are accurate,reproducible and highly precise.This method can be used for quantitative analysis of troxerutin.

关 键 词：UV-VISIBLE SPECTROPHOTOMETRY TROXERUTIN CONTENT determination 

分 类 号：O17[理学—数学]