Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoa  

作　　者：Anne Sofie Rex Chunsen Wu Jorn Aagaard Jens Fedder 

机构地区：[1]Centre of Andrology and Fertility Clinic,Odense University Hospital,Odense 5000,Denmark [2]Aagaard Gynaecological Clinic,Skejby 8200,Aarhus,Denmark [3]Research Unit of Gynaecology and Obstetrics,Faculty of Health,University of Southern Denmark,Odense 5000,Denmark.

出　　处：《Asian Journal of Andrology》2020年第3期246-251,共6页亚洲男性学杂志（英文版）

摘　　要：An increased amount of DNA fragme ntation in the spermatozoa(SDF)is linked to male in fertility.The Sperm Chromati n Structure Assay(SCSA)is widely used for analysis of SDF.However,the current software(SCSASoftR)linked to this assay is licensed and often located within larger diagnostic centers.In this study,we present a protocol for using other types of software than SCSASoftR to determine the SDF index(DFI)with clinical relevance.This protocol is engineered after collect!ng and analyzing 254 samples from fertility patients and sperm donors over a 15-month period.DFI is analyzed using a strict protocol where the spermatozoa are treated with a strong acid(pH 1.2)followed by acridine orange.DFI is determined by a standard flow cytometric software,FACSDiva 6.1.3.Analysis of the outcome of the fertility treatment is included for 137 patients receiving either intrauterine inseminations(IUI)or timed coitus(TC).The results show that the chance of pregnancy decli nes as DFI in creases.We also found that the male DFI affects the chanee of pregnancy independent of the female age.We have shown that a standard flow cytometric software can be used when determi ning a clinical releva nt DFI.These findings are a sign ificant step toward impleme nting the an alysis as a part of the routi ne,in・house diag no sing of the male fertility patient and subseque ntly optimizing the treatme nt course of the couple with reduced human and financial costs.

关 键 词：DNA fragme ntation flow cytometry in-house an alysis in trauteri ne in semi natio n Sperm Chromati n Structure Assay SPERMATOZOA 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]