基于基因芯片探讨花椒影响消化系统功能的作用机制  

Effect mechanism of Zanthoxyli pericarpium on digestive system function based on gene chip

作　　者：高宁[1] 匡海学[1] 孟永海[1] 王知斌[1] 程玉鹏[1] 王秋红 GAO Ning;KUANG Hai-xue;MENG Yong-hai;WANG Zhi-bin;CHENG Yu-peng;WANG Qiu-hong(Key Laboratory of Northern Medicine Base and Application Under Ministry of Education,Heilongjiang Provincial Key Laboratory of Pharmacodynamic Material Bases of Traditional Chinese Medicine and Natural Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Chinese Materia Medica College,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]黑龙江中医药大学,北药基础与应用研究教育部重点实验室,黑龙江省中药及天然药物药效物质基础研究重点实验室,哈尔滨150040 [2]广东药科大学中药学院,广州510006

出　　处：《中华中医药杂志》2020年第3期1187-1191,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)(No.2013CB531801);国家自然科学基金青年科学基金项目(No.81503271);黑龙江省普通本科高等学校青年创新人才培养计划(No.UNPYSCT-2017217);国家自然科学基金面上项目(No.81573539)。

摘　　要：目的:揭示花椒治疗消化系统疾病可能的分子机制。方法:利用基因芯片技术检测花椒给药组与对照组大鼠肝脏基因表达差异,随后采用生物信息学手段分析差异基因所涉及的信号与代谢通路、生物学功能并构建分子相互作用网络。结果:共筛选得到1429条差异基因,富集于内皮素-1(ET-1)信号、血管内皮生长因子(VEGF)信号、多巴胺受体信号等信号通路,涉及癌症、便秘、腹泻、高胃泌素血症等多个消化系统疾病。构建得到1个消化系统疾病相关分子作用网络,其对核因子κB(NF-κB)信号通路的调节起主要作用。结论:花椒能够通过多种途径调节机体消化系统功能,实现养胃健胃、抗癌、抗溃疡等功效。Objective:To investigate the molecular mechanism of curing digestive system diseases by Zanthoxyli pericarpium.Methods:Gene chip was used to detect the differentially expressed genes(DEGs)of livers between rats treated by Zanthoxyli pericarpium and control.Then the data were uploaded to IPA and analysed for investigating the signaling pathway,bio-function as well as molecular interaction network.Results:A total of 1429 DEGs were filtrated,and analysis results showed endothelin-1 signaling,VEGF family ligand-receptor interactions and dopamine receptor signaling were significant enriched,meanwhile the DEGs were related to cancer,constipation,diarrhea,hypergastrinemia,and so on.The interaction network showed NF-κB signaling was the most important.Conclusion:Zanthoxyli pericarpium can regulate the function of digestive system by a variety pathway,consequently nourish the stomach,anti-tumor,anti-ulcer and so on.

关 键 词：花椒 基因芯片 胃溃疡 生物信息 消化系统 分子机制 

分 类 号：R285.5[医药卫生—中药学]