马钱子生物碱单体化合物的体外抗肿瘤活性  

Anti-tumor activity in vitro of nux vomica alkaloid monomer compound

作　　者：孟霜[1] 冯振宇[1] 周晓荣 马小娟 赵建平[1] MENG Shuang;FENG Zhen-yu;ZHOU Xiao-rong;MA Xiao-juan;ZHAO Jian-ping(Shanxi Hospital of Integrated Traditional and Western Medicine,Taiyuan 030013,China;Shanxi University of Chinese Medicine,Taiyuan 030619,China)

机构地区：[1]山西中医学院中西医结合医院,太原030013 [2]山西中医药大学,太原030619

出　　处：《中华中医药杂志》2020年第3期1191-1193,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家国际科技合作专项项目(No.2014DFA33150)。

摘　　要：目的:研究马钱子生物碱单体对人结肠癌细胞HT-29活性的影响。方法:采用MTT法检测马钱子碱、伪马钱子碱、异马钱子碱、伪士的宁、16-甲氧基原士的宁5种化合物对HT-29细胞的抑制能力,并计算IC50。结果:5种生物碱单体化合物对人结肠癌细胞HT-29均表现出不同浓度的生长抑制作用,随着浓度的升高,抑制率呈增长的趋势。马钱子碱、异马钱子碱、伪士的宁、16-甲氧基原士的宁、伪马钱子碱的IC50值分别为0.172、0.227、0.208、0.254、0.262μmol/mL。结论:马钱子碱对结肠癌细胞HT-29的抑制作用最强,其次是伪士的宁、异马钱子碱、16-甲氧基原士的宁、伪马钱子碱。Objective:To study the anti-tumor activity of nux vomica alkaloid monomer compound on human colon cancer cell lines HT-29.Methods:The HT-29 cells were treated with nux vomica alkaloid,pseudobrucine,isobrucine,pseudostrychnine and 16-methoxyl-protostrychnine to obtain inhibitory effect and IC50 by MTT assay.Results:All of the five alkaloids showed different inhibitory effect on HT-29 cells with different concentrations,and the inhibition rates were increased with the concentration increased.The IC50 values of nux vomica alkaloid,isobrucine,pseudostrychnine,16-methoxylprotostrychnine and pseudobrucine,were 0.172,0.227,0.208,0.254,0.262μmol/mL,respectively.Conclusion:Nux vomica alkaloid was demonstrated the strongest inhibitory effects on HT-29 cells,followed by pseudostrychnine,isobrucine,16-methoxylprotostrychnine,pseudobrucine.

关 键 词：马钱子碱 伪马钱子碱 异马钱子碱 伪士的宁 16-甲氧基原士的宁 抗肿瘤 MTT 

分 类 号：R285.5[医药卫生—中药学]