RP-HPLC法测定不同产地草龙中3种三萜类成分的含量  

Determination analysis of three triterpenses constituents in different habitats of Ludwigia hyssopifoLia(G.Don)Exell by RP-HPLC

作　　者：卢汝梅[1] 张进燕 赵惠玲[1] 王肖 韦建华[1] 李兵[1] LU Ru-mei;ZHANG Jin-yan;ZHAO Hui-ling;WANG Xiao;WEI Jian-hua;LI Bing(Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西中医药大学,南宁530001

出　　处：《中华中医药杂志》2020年第3期1472-1475,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西壮族自治区食品药品监督管理局项(计划内项目);广西科技厅重大专项计划课题项目(No.桂科重1355001-4-1);广西高校中药提取纯化与质量分析重点实验室(No.桂教科研[2014]6号);广西中医药大学2019年校级重点硕士研究生科研创新项目(No.YCSZ20190014)。

摘　　要：目的:对草龙Ludwigia hyssopifoLia(G.Don)Exell药材的质量控制进行研究,为草龙质量标准的制定提供科学依据。方法:采用HP L C法定量测定其中2α,3β,19α,6β,24-五羟基-12-烯-18-乌苏酸、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸及乌发醇的含量,以COSMOSIL 5C18-PAQ(4.6mm×250mm,5μm)为色谱柱;流动相:乙腈(B)-水(A),梯度洗脱(0~18min、29%B;18~45min、53%B);体积流量:1mL/min;检测波长:210nm;柱温:30℃。结果:2α,3β,19α,6β,24-五羟基-12-烯-18-乌苏酸、2α,3β,19α,23-四羟基-12-烯-28-乌苏酸及乌发醇分别0.0499~0.7982μg(r=0.9995)、0.2000~3.1997μg(r=0.9999)、0.5001~8.0019μg(r=0.9997)线性关系良好,平均回收率分别为100.43%(RSD=1.43%)、100.03%(RSD=1.24%)、100.04%(RSD=1.47%)。结论:该方法准确、可靠、重复性好,可用于草龙药材的质量控制。Objective:To study the quality control of Ludwigia hyssopifoLia(G.Don)Exell,and to provide scientific basis for the formulation of the quality standards.Methods:The content of 2α,3β,19α,6β,24-pentahydroxylurs-12-ene-18-ursolic acid,2α,3β,19α,23-tetrahydroxyurs-12-ene-28-ursolic acid and ufactol were determined by HPLC.COSMOSIL 5C18-PAQ(4.6mm×250mm,5μm)was used as the column;mobile phase:acetonitrile(B)-water(A),gradient elute(0~18min,29%B;18~45min,53%B),the flow rate was 1mL/min,the detection wavelength was 210nm and the temperature at 30℃.Results:2α,3β,19α,6β,24-pentahydroxylurs-12-ene-18-ursolic acid,2α,3β,19α,23-tetrahydroxyurs-12-ene-28-ursolic acid and ufactol have good linear relationships in the range of 0.0499~0.7982μg(r=0.9995),0.2000~3.1997μg(r=0.9999)and 0.5001~8.0019μg(r=0.9997)respectively.And the average recoveries were 100.43%,100.03%and 100.04%;RSD were 1.43%,1.24%,1.47%,respectively.Conclusion:The method is accurate,reliable and repeatable,and can be used for the quality control of Ludwigia hyssopifoLia(G.Don)Exell.

关 键 词：草龙 2α 3β 19α 6β 24-五羟基-12-烯-18-乌苏酸 2α 3β 19α 23-四羟基-12-烯-28-乌苏酸 乌发醇 高效液相色谱 三萜类成分 含量测定 

分 类 号：R284.1[医药卫生—中药学]