超高效液相色谱法检测土壤中硝基呋喃类药物残留  

Determination of nitrofurans residues in soil by ultra performance liquid chromatography

作　　者：乌仁图雅[1] 邓宇[1] 荣迪 王岩[1] 娜日娜[1] 陈琪[1] 赵剑平[1] 正月 Wurentuya;DENG Yu;RONG Di;WANG Yan;Narina;CHEN Qi;ZHAO Jianping;ZHENG Yue(Chifeng Academy of Agriculture and Animal Husbandry,Inner Mongolia,Chifeng 024031,China)

机构地区：[1]内蒙古赤峰市农牧科学研究院,内蒙古赤峰024031

出　　处：《化学分析计量》2020年第3期21-25,共5页Chemical Analysis And Meterage

基　　金：国家农业基础性长期性科技工作(ZX03S0509)。

摘　　要：建立超高效液相色谱法检测土壤中硝基呋喃类药物残留。通过单因素和正交设计试验,考察了超声时间、提取温度、提取次数对硝基呋喃类药物残留回收率的影响。最优提取条件为超声时间10 min、提取温度45℃、提取次数为4次。硝基呋喃类药物的质量浓度在1~4μg/mL范围内与色谱峰面积成良好的线性关系,线性相关系数均大于0.9999。加标回收率为74.34%~102.52%,测定结果的相对标准偏差为0.91%~4.49%(n=6)。该方法简单、快速,测定结果准确可靠,可用于土壤中硝基呋喃类药物残留量的测定。A method for the determination of nitrofurans residues in soil by ultra performance liquid chromatography was established.The effects of ultrasonic time,extraction temperature,and number of extractions on the recovery of nitrofuran residues were investigated by single-factor and orthogonal design experiments.The optimal extraction conditions:the ultrasonic time was 10 min,the extraction temperature was 45℃,and extraction times was 4.The mass concentration of nitrofurans had good linear relationship with the chromatographic peak area in the range of 1-4μg/mL,the linear correlation coefficients were all more than 0.9999.The spike recovery rate was 74.34%-102.52%,and the relative standard deviation of the measurement results was 0.91%-4.49%(n=6).The method is simple,fast,and accurate,which can be used for the determination of nitrofuran residues in soil.

关 键 词：土壤 硝基呋喃类药物 超高效液相色谱法 

分 类 号：O657.7[理学—分析化学]