HPLC法测定条叶旋覆花不同部位中线叶旋覆花内酯A的含量  

Determination of britanin content in different parts of Inula linariifolia Turcz by HPLC

作　　者：李玉斌 叶霁 张卫东 LI Yubin;YE Ji;ZHANG Weidong(Department of Natural Medicinal Chemistry,School of Pharmacy,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学药学院天然药物化学教研室,上海200433

出　　处：《药学实践杂志》2020年第3期245-249,共5页Journal of Pharmaceutical Practice

基　　金：“长江学者奖励计划”项目;国家自然科学基金项目(81520108030,21472238);上海市活性天然产物制备工程技术研究中心项目(16DZ2280200);上海市科委中药现代化专项(13401900103,13401900101);国家重点研发计划(2017YFC1700200)。

摘　　要：目的利用HPLC法对7批不同产地金沸草基源植物之一条叶旋覆花的干燥地上部分中线叶旋覆花内酯A的含量进行测定,并与其在头状花序中的含量比较,为金沸草药材质量标准的制定及合理应用提供依据。方法Agilent Zorbax SB C18色谱柱(250 mm×4.6 mm,5μm),在柱温为30℃、流速为1.2 ml/min下以乙腈-水为流动相梯度洗脱,检测波长为212 nm。结果实验结果符合《中华人民共和国药典》(2015版)方法学验证的要求,金沸草基源条叶旋复花的头状花序中线叶旋覆花内酯A的平均含量为0.732%,在干燥地上部分的平均含量为0.125%,仅为头状花序的1/7,差异显著。结论建立的质量控制方法简单易行,可为条叶旋覆花的质量控制及合理用药提供数据支持和科学依据。Obejctive To determine and compare britanin content in dried aerial parts and capitulum of Inula linariifolia Turcz from 7 different origins by HPLC.Methods Analysis was performed on Agilent Zorbax SB C18(250 mm×4.6 mm,5μm).Acetonitrile and water were used as mobile phase for gradient elution at 1.2 ml/min.Column temperature was 30℃and the detection wavelength at 212 nm.Results The results meet the requirements of the method validation in 2015 edition of Chinese Pharmacopeia.The average britanin content in dried aerial parts of Inula linariifolia Turcz is 0.125%vs 0.732%in capitulum,which is significantly different.Conclusion The established method is simple and convenient.It can be used for quality control of Inula linariifolia Turcz.

关 键 词：条叶旋覆花 干燥地上部分 头状花序 线叶旋覆花内酯A 含量测定 

分 类 号：R284.1[医药卫生—中药学]