弱稳恒磁场环境下基于BiFeO3@TiO2复合纳米颗粒体外光动力疗法灭活HL60细胞试验研究  

An Experiment Study on Inactivation of HL60 Cells by BiFeO3@TiO2 Composite Nanoparticle Photodynamic Therapy in vitro under Weak Stable Magnetic Field

作　　者：赵杨 刘丽玲 张启云 方杰 陈丽[2] 艾保全[1] 熊建文[1] ZHAO Yang;LIU Liling;ZHANG Qiyun;FANG Jie;CHEN Li;AI Baoquan;XIONG Jianwen(School of Physics and Telecommunication Engineering,South China Normal University,Guangzhou 510006,China;School of Physics and Optoelectronic Engineering,Guangdong University of Technology,Guangzhou 510006,China)

机构地区：[1]华南师范大学物理与电信工程学院,广州510006 [2]广东工业大学物理与光电工程学院,广州510006

出　　处：《激光生物学报》2020年第2期128-134,152,共8页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(61072029);广州市科技计划项目(2014J4100049)。

摘　　要：本文通过溶胶-凝胶法制备了BiFeO3@TiO2复合纳米颗粒,利用透射电镜(TEM)、X射线衍射(XRD)、荧光发光光谱等对纳米颗粒进行表征。研究采用Cell Counting Kit-8(CCK-8)法分别检测了在暗室条件、光照条件以及不同强度的弱稳恒磁场作用下,用终值质量浓度为50μg/mL纳米颗粒处理HL60细胞活性,试验结果表明:在暗室条件下,药物质量浓度为50μg/mL,与HL60细胞共同孵育12 h后,BiFeO3@TiO2复合纳米颗粒随着TiO2外壳厚度增加,细胞的暗室相对存活率从78%增加到85%;在光照条件下,有弱稳恒磁场作用的BiFeO3与TiO2质量比为1∶2的BiFeO3@TiO2复合纳米颗粒对HL60细胞的PDT灭活效率最高达到78%,弱稳恒磁场环境增强了对HL60细胞的PDT灭活效率,这为对弱稳恒磁场环境下的光动力疗法治疗白血病肿瘤细胞的临床应用提供了参考。In this paper,BiFeO3@TiO2 composites nanoparticles were synthesized by sol-gol method.The nanoparticles were characterized by transmission electron microscope(TEM),X-ray diffraction(XRD),fluorescence emission spectrum etc.The Cell Counting Kit-8(CCK-8)method was used to detect the activity of HL60 cells treated with a final concentration of 50μg/mL nanoparticles under the action of weak stable magnetic field with different strengths in dark room and light conditions.The experimental results showed that the viability of HL60 cells treated with BiFeO3@TiO2 composites nanoparticles for 12 hours at a concentration of 50μg/mL increased from 78%to 85%,with the increment of TiO2 shell thickness in the darkroom condition.However,cell groups treated by BiFeO3@TiO2 composites nanoparticles with a mass ratio of BiFeO3 to TiO2 of 1∶2 showed the highest cell inactivated efficiency of 78%under light exposure.The weak stable magnetic field enhanced the PDT inactivation efficiency of HL60 cells.Therefore,it provides a reference for the clinical application of photodynamic therapy in the treatment of tumor cells under weak stable magenetic field.

关 键 词：BiFeO3@TiO2复合纳米颗粒 弱稳恒磁场 光动力疗法 HL60细胞 

分 类 号：R454.2[医药卫生—治疗学]