VC发酵废液投喂褶皱臂尾轮虫的可行性研究  

Potentiality of cultivation of rotifer Brachionus plicatilis with vitamin C fermentation wastes

作　　者：刘鑫 王伟 姜玉声 冯思雨 何雨航 曹林泉 陈雪 LIU Xin;WANG Wei;JIANG Yusheng;FENG Siyu;HE Yuhang;CAO Linquan;CHEN Xue(College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023

出　　处：《大连海洋大学学报》2020年第3期333-338,共6页Journal of Dalian Ocean University

基　　金：辽宁省高等学校创新人才计划项目;辽宁省重点研发计划项目(2018203004);大连市杰出青年科技人才项目(2017RJ10);大连海洋大学湛蓝学者项目;辽宁省大学生创新创业项目;大连海洋大学大学生创新创业项目。

摘　　要：为研究利用制备维生素C所产生的发酵废液培养褶皱臂尾轮虫Brachionus plicatilis的可行性,并制定合理的投喂方案,在温度为(29.0±1.4)℃、盐度为23.0±1.1的海水中接种轮虫,密度为20 ind./mL,分别用微拟球藻(A)、发酵废液(B)、裂殖壶藻(C)、微拟球藻+发酵废液(D)、微拟球藻+裂殖壶藻(E)、微拟球藻+发酵废液+裂殖壶藻(F)、微拟球藻+发酵废液固型物(G)7种饵料投喂7组轮虫,每组设3个平行,连续培养7 d。结果表明:混合投喂微拟球藻+发酵废液固型物的G组轮虫密度最高,为211 ind./mL,其抱卵率、日均增长量、日均增长率均最高,分别为(69.4±7.1)%、(27.3±4.6)ind./(mL·d)和(33.5±2.6)%/d;只投喂发酵废液的B组轮虫密度最低,为11 ind./mL;各组轮虫密度依次为G>E>D>F>A>C>B;饵料中含有发酵废液的B、D、G组水中总氨氮、亚硝酸盐和硝酸盐浓度均较高,其中,仅投喂发酵废液的B组氨氮浓度最高,为13.81 mg/L;各组水体中pH均有下降,其中B、D组下降趋势明显,试验结束时分别降至7.13、7.17,而C组全程无显著性变化。研究表明,以制备VC的发酵废液培养海水褶皱臂尾轮虫具有可行性,建议施用前将其进行固液分离,配合微藻等其他饵料混合投喂。Rotifer Brachionus plicatilis was cultured in a 15 L glass jar at a density of 20 ind./mL under continuous aeration and fed 7 kinds of diets including alga Nannochloropsis sp.(A),vitamin C fermentation waste(B),alga Schizochytrium sp.(C),alga Nannochloropsis sp.+vitamin C fermentation waste(D),alga Nannochloropsis sp.+Schizochytrium sp.(E),Nannochloropsis sp.+vitamin C fermentation waste+Schizochytrium sp.(F),and Nannochloropsis sp.mixed with solid fermentation waste(G)at water temperature of(29.0±1.4)℃,and salinity of 23.0±1.1 for 7 days to evaluate the potentiality of rotifer cultivation with vitamin C fermentation wastes.The maximal density(211 ind./mL)of the rotifer was found in the group(G),with the maximal carrying egg rate of(69.4±7.1)%,average daily production of(27.3±4.6)ind./(mL·d)and average daily growth rate of(33.5±2.6)%/d and the minimal density(11 ind./mL)of the rotifer in group B.Descending order of the rotifer density was expressed as group G>group E>group D>group F>group A>group C>group B.There were high concentrations of ammonia nitrogen,nitrite,and nitrite in group B,group D and group G,containing fermentation waste,with the maximal concentration of ammonia nitrogen(13.81 mg/L)in group B.The pH values were found to be decreased in all the groups,especially in group B(7.13)and group D(7.17)at the end of the experiment,without obvious change.In conclusion,vitamin C fermentation waste is a potential food for rotifer culture,and it is recommended that the vitamin C fermentation waste be fed as solid mass without liquid containing high concentration of ammonia nitrogen,and mixed with other food such as microalgae.

关 键 词：褶皱臂尾轮虫 发酵废液 生长 水质 

分 类 号：S917[农业科学—水产科学]