枯草芽孢杆菌对断奶仔猪生长性能和肠道形态、黏膜免疫及菌群数量的影响  

Effects of Bacillus subtilis on Growth Performance,Intestinal Morphology,Mucosal Immune and Microflora Number of Weaned Piglets

作　　者：邢帅兵 陈代文[1] 余冰[1] 栾超 何军[1] 黄志清[1] 毛湘冰[1] 郑萍[1] 罗钧秋[1] 罗玉衡[1] 虞洁[1] XING Shuaibing;CHEN Daiwen;YU Bing;LUAN Chao;HE Jun;HUANG Zhiqing;MAO Xiangbing;ZHENG Ping;LUO Junqiu;LUO Yuheng;YU Jie(Key Laboratory of Animal Disease Resistance and Nutrition of Ministry of Education,Key Laboratory of Animal Disease Resistance and Nutrition of Sichuan Province,College of Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu 611130,China;Adisseo Life Science(Shanghai)Co.,Ltd.,Shanghai 201200,China)

机构地区：[1]四川农业大学动物营养研究所,动物抗病营养教育部重点实验室,动物抗病营养四川省重点实验室,成都611130 [2]安迪苏生命科学制品(上海)有限公司,上海201200

出　　处：《动物营养学报》2020年第5期2066-2073,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：“十三五”国家重点研发计划专项(2016YFD0500506);国家生猪产业技术体系(CARS 35)。

摘　　要：本试验旨在研究饲粮中添加枯草芽孢杆菌替代饲用抗生素对断奶仔猪生长性能和肠道健康的影响。试验选用21日龄杜长大(DLY)断奶仔猪72头,随机分为4组,分别为对照组(CON组)、抗生素组(ANT组)、抗生素+枯草芽孢杆菌组(ANT+BS组)、枯草芽孢杆菌组(BS组),每组6个重复,每个重复3头猪。试验期49 d。结果表明:1)试验全期,各组间平均日增重和平均日采食量均无显著差异(P>0.05),与CON组相比,仅ANT组的料重比显著降低(P<0.05)。2)各组间十二指肠、空肠、回肠的绒毛高度、隐窝深度、绒毛高度/隐窝深度均无显著差异(P>0.05);与CON组相比,ANT组、ANT+BS组和BS组回肠的隐窝深度分别降低了10.16%、14.98%、18.10%,有差异显著的趋势(P=0.084)。3)与CON组相比,ANT+BS组和BS组盲肠中双歧杆菌的数量显著提高(P<0.05),且与ANT组无显著差异(P>0.05)。4)与CON组相比,ANT+BS组和BS组空肠黏膜中分泌型免疫球蛋白A的含量显著提高(P<0.05),但均显著低于ANT组(P<0.05)。综上所述,枯草芽孢杆菌可以改善断奶仔猪小肠形态结构,增加肠道内有益菌的数量,提高小肠黏膜免疫功能,达到部分替代抗生素的效果,并且枯草芽孢杆菌与抗生素联用无协同或拮抗作用。This experiment was conducted to investigate the effects of adding Bacillus subtilis to replace antibi otics on growth performance and intestinal health of weaned piglets.A total of 72 healthy 21 day old DLY weaned piglets were randomly allotted 4 groups with 6 replicates per group and 3 piglets per replicate.The four groups were CON group,ANT group,ANT+BS group and BS group,respectively.The trial lasted for 49 days.The results showed as follows:1)there was no significantly difference in average daily gain(ADG)and average daily feed intake(ADFI)among groups(P>0.05);compared with the CON group,feed/gain(F/G)in ANT group was significantly decreased(P<0.05).2)There was no significantly difference in vil lous height,crypt depth and villous height/crypt depth(VH/CD)of duodenum,jejunum and ileum among groups(P>0.05);compared with the CON group,the crypt depth of ileum in ANT group,ANT+BS group and BS group decreased by 10.16%,14.98%and 18.10%,and with a tendency for significant differences(P=0.084).3)Compared with the CON group,the number of Bifidobacteria in cecum in ANT+BS group and BS group was significantly increased(P<0.05),and there was no significantly difference compared with ANT group(P>0.05).4)The secreted immunoglobulin A(SIgA)content in jejunum mucosa in ANT+BS group and BS group was significantly increased(P<0.05),but was significantly lower than that in ANT group(P<0.05).In summary,B.subtilis can improve the intestinal structure of weaned piglets,increase the number of beneficial bacteria in the intestinal tract,improve the immune function of intestinal mucosa,and achieve the effect of partial substitution of antibiotics,and there is no synergistic or antagonistic effect between B.subtilis and antibiotics.

关 键 词：枯草芽孢杆菌 断奶仔猪 生长性能 肠道形态 肠道菌群 肠道免疫 

分 类 号：S828[农业科学—畜牧学]