注射胰岛素对不同品种鸡血糖浓度和采食情况的影响  

Effects of Insulin Injection on Blood Glucose Concentration and Feeding Status of Different Breed Chickens

作　　者：王焕杰 杜鹏飞 张吉昌 朱瑶 姬杰菲 陈文[1] 黄艳群[1] WANG Huanjie;DU Pengfei;ZHANG Jichang;ZHU Yao;JI Jiefei;CHEN Wen;HUANG Yanqun(Feed Nutrition Engineering Laboratory of Henan Province,Henan Agricultural University,Zhengzhou 450002,China;Nanyang Wancheng District Animal Health Supervision,Nanyang 473000,China)

机构地区：[1]河南农业大学饲料营养河南省工程实验室,郑州450002 [2]南阳市宛城区动物卫生监督所,南阳473000

出　　处：《动物营养学报》2020年第5期2164-2175,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：智汇郑州1125聚才计划——创新领军人才项目。

摘　　要：本试验旨在探究注射胰岛素对不同品种鸡血糖浓度和采食情况的影响。试验1选用21日龄海兰蛋鸡(简称蛋鸡)、丝毛乌骨鸡(简称乌鸡)和爱拔益加(AA)肉鸡(简称肉鸡)雄性雏鸡各120只,随机各分为4组:胰岛素禁食组(n=60,其中12只鸡用来测定血糖浓度的动态变化,其余48只鸡在注射胰岛素后0、15、120和240 min时,每个时间点各屠宰12只鸡,收集颈静脉血液10 mL,用来测定血清胰岛素浓度)、磷酸盐缓冲液(PBS)禁食组(n=12)、胰岛素喂食组(n=24)和PBS喂食组(n=24)。试验前禁食16 h,注射剂量为5 IU/kg的胰岛素或相同剂量的PBS,禁食各组在注射后继续保持禁食,并测定血糖浓度的动态变化;喂食各组在注射后立即恢复喂食,1/2鸡只用于测定血糖浓度的动态变化,1/2鸡只用于统计采食情况。试验2选用44日龄蛋鸡、乌鸡和肉鸡各48只,随机各分为2组:胰岛素组(n=24)和PBS组(n=24),试验前禁食16 h,注射剂量为2.3 IU/kg的胰岛素或相同剂量的PBS,注射120 min后恢复喂食,各组鸡1/2鸡只用于测定血糖浓度的动态变化,1/2鸡只用于统计采食情况。结果表明:1)禁食状态下,注射胰岛素可显著降低21日龄各品种鸡的血糖浓度(P<0.05),且注射后15 min时各品种鸡的血糖浓度均已显著低于基础(0 min)血糖浓度(P<0.05),且持续下降直到120 min时;肉鸡的血糖浓度与蛋鸡和乌鸡相比下降幅度更大,且120 min后血糖浓度恢复能力显著低于蛋鸡和乌鸡(P<0.05);注射胰岛素后肉鸡不同时间点的血清胰岛素浓度均高于蛋鸡和乌鸡。2)与基础血糖浓度相比,21日龄时注射胰岛素后立即恢复喂食显著降低蛋鸡60和120 min时的血糖浓度(P<0.05),显著提高肉鸡30和60 min时的血糖浓度(P<0.05)。3)注射胰岛素后立即恢复喂食对21日龄各品种鸡240 min内的累积采食量无显著影响(P>0.05),显著降低蛋鸡和乌鸡注射后120~240 min时的采食量(P<0.05),显著提高肉鸡注射后30~60 min�This study was conducted to explore the effects of insulin injection on blood glucose concentration and feeding status of different breed chickens.Experiment 1:one hundred and twenty male Hy line laying hens(abbreviated as laying hens),silky black bone chickens(abbreviated as silky chickens)and Arbor Acres(AA)broiler chickens(abbreviated as broiler chickens)at 21 days of age were randomly divided into 4 groups,respectively,and the 4 groups were insulin fasting group(n=60,of which 12 chickens were used to measure dynamic changes of blood glucose concentration,and rest 48 chickens were slaughtered at 0,15,120 and 240 min after insulin injection and 12 chickens per time point,and 10 mL jugular blood were collected to determine serum insulin concentration),phosphate buffered saline(PBS)fasting group(n=12),insulin feeding group(n=24)and PBS feeding group(n=24).All chickens fasted for 16 h before test,injection dose was 5 IU/kg insulin or the same dosage of PBS,chickens in fasting groups were continued to fasted after injection and used to determine dynamic changes of blood glucose concentration,and chickens in feeding groups were feeding immediately after injection,one half were used to measure dynamic changes of blood glu cose concentration and another half were used to statistics feeding status.Experiment 2:forty eight laying hens,silky chickens and broiler chickens at 44 days of age were randomly divided into 2 groups,respectively,insulin group(n=24)and PBS group(n=24).All chickens fasted for 16 h before test,injection dose was 2.3 IU/kg insulin or the same dosage of PBS,and feeding after 120 min of injection,one half in each group were used to measure dynamic changes of blood glucose concentration,and another half were used to statistics feeding statues.The results showed as follows:1)insulin injection significantly decreased blood glucose con centration of all breed chickens at 21 days of age in fasting state(P<0.05).Blood glucose concentration of all breed chickens was significantly lower than basic blood glucos

关 键 词：蛋鸡 乌鸡 肉鸡 胰岛素 血糖浓度 采食 

分 类 号：S831[农业科学—畜牧学]