木质素降解复合菌的选育及其对芦苇浆卡伯值的影响  

Screening of lignin degradation compound strains and its effect on kappa value of reed pulping

作　　者：王蕾[1,2,3] 卢庆华[3] 张俊[3] 石碧瑶 廖祥儒 Wang Lei;Lu Qinghua;Zhang Jun;Shi Biyao;Liao Xiangru(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China;Inner Mongolia Key Laboratory of Biomass-Energy Conversion,Inner Mongolia University of Science and Technology,Baotou 014010,China)

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122 [3]内蒙古科技大学内蒙古自治区生物质能源化利用重点实验室,内蒙古包头014010

出　　处：《河南师范大学学报（自然科学版）》2020年第3期103-109,共7页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31360126);内蒙古自治区自然科学基金(2015MS0303).

摘　　要：筛选木质素降解菌株并从中挑出产漆酶(Lac)和锰过氧化物酶(MnP)能力较强的4株菌株,复合培养得到4种复合菌ABC,ACD,ABD和BCD.复合培养后Lac和MnP产酶高峰较单一培养均有所提前,ABC,ACD的Lac和MnP酶活力活在60~72 h达到最大.BCD的Lac和MnP酶活力均高于其他3种,而且其MnP有两个产酶高峰.芦苇浆经4种复合菌处理后,卡伯值均有所下降,经BCD处理后卡伯值降幅最大,达到(5.3±0.12).Lignin degradation strains were screend and 4 strains with high laccase-producing activity and manganese peroxidases-producing activity were selected.After compound culture,4 kinds of compound strains ABC,ACD,ABD and BCD were obtained.The enzyme production peaks of Lac and MnP in compound culture were earlier than that in single culture.The enzyme activity of Lac and MnP produced by strain ABC and ACD reached the maximum in 60-72 h.The activity of Lac and MnP produced by strain BCD were higher than the others,and there are two enzyme production peaks of its MnP.After treatment by compound strains,the kappa number of reed pulping were decreased,among which the kappa number decreased the most after the treatment by strain BCD,reaching 5.3±0.12.

关 键 词：木质素降解 漆酶 锰过氧化物酶 卡伯值 

分 类 号：Q819[生物学—生物工程]