四神丸中3种成分在正常大鼠及抑郁大鼠体内药动学的比较  

Comparison of in vivo pharmacokinetics of three constituents in Sishen Pills in normal rats and depressed rats

作　　者：孙红 宋珊珊 井汶 张优 谷雨[1] 居文政[1] SUN Hong;SONG Shan shan;JING Wen;ZHANG You;GU Yu;JU Wen zheng(The Hospital Affiliated to Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院,江苏南京210029

出　　处：《中成药》2020年第5期1109-1114,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(81573685)。

摘　　要：目的 比较四神丸中3种成分在正常大鼠及抑郁大鼠体内的药动学.方法 12只大鼠随机分为正常组和模型组,每组6只,灌胃给予四神丸混悬液(4.63 mL/kg),于5、15、30、45、60、90、120、180、240、360、480、720、960、1 440 min采血,LC-MS/MS法测定补骨脂素、异补骨脂素、五味子醇甲血药浓度,绘制血药浓度-时间曲线,计算主要药动学参数.结果 模型组3种成分Cmax、 AUC0~t、AUC0~∞及补骨脂素tmax高于正常组(P<0.05,P<0.01),2组3种成分t1/2比较差异无统计学意义(P>0.05).结论 四神丸中补骨脂素、异补骨脂素、五味子醇甲在抑郁大鼠体内的吸收程度高于正常大鼠体内,而消除程度无明显变化.AIM To compare the in vivo pharmacokinetics of three constituents in Sishen Pills in normal rats and depressed rats.METHODS Twelve rats were randomly assigned into normal group and model group,6 rats in each group,all of which were given intragastric administration of Sishen Pills suspension(4.63 mL/kg).Blood collections were performed at 5,15,30,45,60,90,120,180,240,360,480,720,960,1440 min,LC MS/MS was adopted in the plasma concentration determination of psoralen,isopsoralen and schisandrin A,plasma concentration time curves were drawn,and main pharmacokinetic parameters were calculated.RESULTS The Cmax,AUC0-t,AUC0-∞of three constituents and tmax of psoralen in the model group were higher than those in the normal group(P<0.05,P<0.01),and the t1/2 of three constituents in the two groups demonstrated no obvious differences(P>0.05).CONCLUSION Psoralen,isopsoralen and schisandrin A in Sishen Pills display higher absorption degrees in depressed rats in vivo than those in the normal rats in vivo,while elimination degrees exhibit no obvious changes.

关 键 词：四神丸 补骨脂素 异补骨脂素 五味子醇甲 抑郁症 LC-MS/MS 

分 类 号：R969.1[医药卫生—药理学]