丝棉木种子化学成分及其抗肿瘤活性  

Chemical constituents from the seeds of Euonymus maackii and their antitumor activities

作　　者：庞策 左亚奇 张冠腾 甄攀[2] PANG Ce;ZUO Ya qi;ZHANG Guan teng;ZHEN Pan(Department of Pharmacy,Hebei North University,Zhangjiakou 075000,China;Institute of Applied Chemistry,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院药学系,河北张家口075000 [2]河北北方学院应用化学研究所,河北张家口075000

出　　处：《中成药》2020年第5期1208-1214,共7页Chinese Traditional Patent Medicine

基　　金：河北省高等学校科学技术研究项目(ZD2016016);2018年度河北北方学院一般项目(YB2018030)。

摘　　要：目的 研究丝棉木Euonymus maackii Rupr.种子的化学成分及其抗肿瘤活性.方法 丝棉木种子95%乙醇提取物乙酸乙酯部位采用硅胶、D101、反相HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.MTT法测定其抗肿瘤活性.结果 从中分离得到10个化合物,分别鉴定为1β-甲基正丁酰氧基-2β-苯甲酰氧基-4α-羟基-6α-呋喃酰氧基-9β,12-二乙酰氧基-β-二氢沉香呋喃(1)、6α,9β,12-三乙酰氧基-1β,2β,8β-三苯甲酰氧基-β-二氢沉香呋喃(2)、6α,9β,12-三乙酰氧基-1β,8β-二苯甲酰氧基-2β-正己酰氧基-β-二氢沉香呋喃(3)、6α,9β,12-三乙酰氧基-1β,8β-二苯甲酰氧基-2β-正辛酰氧基-β-二氢沉香呋喃(4)、6α,9β,12-三乙酰氧基-1β,8β-二苯甲酰氧基-2β-正癸酰氧基-β-二氢沉香呋喃(5)、卫矛羰碱(6)、6α,12-二乙酸基-1β,9α-二乙酸(β-呋喃羧氧基)-4α-羟基-2β-2-甲基丁酯-β-二氢沉香呋喃(7)、6α,9β,12-三乙酰氧基-1β,8β-二苯甲酰氧基-2β-羟基-β-二氢沉香呋喃(8)、6α,12-二乙酸基-1β,9 α-二乙酸(β-呋喃羧氧基)-4α-羟基-1β-2-甲基丁酯-β-二氢沉香呋喃(9)、6α,9β,12-四乙酰氧基-1β,8β-二苯甲酰氧基-β-二氢沉香呋喃(10).化合物6对Hela细胞有明显的抑制作用,IC50为9.76 μg/mL.结论 化合物1为新化合物.化合物6有一定的抗肿瘤活性.AIM To study the chemical constituents from the seeds of Euonymus maackii Rupr.and their anti tumor activities.METHODS The ethyl acetate fraction of 95%ethanol extract from the seeds of E.maackii was isolated and purified by silica,D101 and reversed phase HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor activities were determined by MTT.RESULTS Ten compounds were isolated and identified as 1β methyln butyryloxy 2β benzoyloxy 4α hydroxy 6α furanyloxy 9β,12 diacetyloxy β dihydroagarofuran(1),6α,9β,12 triacetyloxy 1β,2β,8β tribenzoyloxy β di hydroagarofuran(2),6α,9β,12 triacetyloxy 1β,8β dibenzoyloxy 2β N hexaneacyl β dihydroagarofuran(3),6α,9β,12 triacet yloxy 1β,8β dibenzoyloxy 2β N octanoyl β dihydroagarofuran(4),6α,9β,12 triacetyloxy 1β,8β diben zoyloxy 2β N decanyl β dihydroagarofuran(5),evonine(6),6α,12 diacetoxy 1β,9αdi(β furancarbonyl oxy) 4α hydroxy 2β 2 methylbutanoyloxy β dihydroagarofuran(7),6α,9β,12 triacetyloxy 1β,8β dibenzoy loxy 2β hydroxy β dihydroagarofuran(8),6α,12 diacetoxy 1β,9αdi(β furancarbonyloxy) 4α hydroxy 1β 2 methylbutanoyloxy β dihydroagarofuran(9),6α,9β,12 tetraacetyl 1β,8β dibenzoyloxy β di hydroagarofuran(10).Compound 6 had an obvious inhibitory effect on Hela cells with the IC50 value of 9.76μg/mL.CONCLUSION Compound 1 is a new compound.Compound 6 has certain antitumor activity.

关 键 词：丝棉木 种子 化学成分 分离鉴定 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]