基于四逆汤建立附子炮制新方法  

Establishment of a new method of processing Aconiti Lateralis Radix Praeparata based on Sini Decoction

作　　者：陈雪 王冬阁 冯正平 李嘉欣 张国彦 刘志超 康杰芳 崔浪军 CHEN Xue;WANG Dong ge;FENG Zheng ping;LI Jia xin;ZHANG Guo yan;LIU Zhi chao;KANG Jie fang;CUI Lang jun(National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China,the Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry,The Ministry of Education,College of Life Sciences,Shaanxi Normal University,Xi’an 710119,China;Chenggu County Qunli Traditional Chinese Medicine Cooperative,Hanzhong 723200,China)

机构地区：[1]陕西师范大学生命科学学院,药用资源与天然药物化学教育部重点实验室,西北濒危药材资源开发国家工程实验室,陕西西安710062 [2]城固县群利中药材合作社,陕西汉中723200

出　　处：《中成药》2020年第5期1255-1261,共7页Chinese Traditional Patent Medicine

基　　金：科技部科技惠民计划(2012GS610102,2013GS610202);陕西省科技攻关计划(2016SF 376);陕西师范大学中央高校基本科研业务费(GK201603110);陕西省重点产业创新链(群)项目(2019ZDLSF04 01 0);2017年中医药公共卫生服务补助专项(财社[2017]66)。

摘　　要：目的 在四逆汤的基础上建立附子炮制新方法.方法 附子饮片25%氨水提取物的分析采用Phenomenex RPC18色谱柱(250 mm×4.6 mm,5μm);流动相0.1%二乙胺-乙腈,梯度洗脱;柱温25℃;体积流量1.0 mL/min;检测波长230 nm.分别考察炮制过程中所用不同生物酶比例及用量、甘草与干姜的用量以及蒸制时间对附片生物碱含有量的影响,进而筛选出最优的炮制工艺.结果 6种生物碱在各自范围内线性关系良好(r>0.997 5),平均加样回收率91.51%~ 102.3%,RSD 0.90%~2.41%.在炮制过程中,加入混合酶,随后使用甘草粉-干姜粉的混合粉均匀挂粉后进行高压蒸制40 min,得到效毒比最高的新型附片.此新型附片的苯甲酰新乌头原碱含有量可达0.035%,而双酯型生物碱总量低至0.010%.结论 该方法简便易行可控,所得附片有效且可安全使用,可作为替代附子传统加工工艺的新方法.AIM To establish a new method of processing Aconiti Lateralis Radix Praeparata based on Sini De coction.METHODS The analysis of 25%ammonia extract from Aconiti Lateralis Radix Praeparata was developed on a 25℃thermostatic Phenomenex RP C18column(250 mm×4.6 mm,5μm),with the mobile phase comprising of 0.1%diethylamine acetonitrile flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.An optimal process was screened out.The parameters included the proportion and dosage of enzymes,the dosage of liquorice powder and ginger powder,and the steaming time.RESULTS Six al kaloids showed good linear relationships within their own ranges(r>0.9975),whose average recoveries were 91.51%-102.3%,with the RSDs of 0.90%-2.41%.After treating with mixed enzyme powder,Aconiti Lateralis Radix Praeparata was mixed with glycyrrhiza and ginger powder,then steamed at high pressure for 40 minutes.In the new Aconiti Lateralis Radix Praeparata processed pieces,the content of benzoylmes aconine was 0.035%,and the total amount of diester alkaloids was as low as 0.010%.CONCLUSION The simple and available method can be used as a new method for replacing the traditional processing technology of Aconiti Lateralis Radix Praeparata.

关 键 词：附子 甘草 干姜 生物碱 四逆汤 HPLC 

分 类 号：R283.3[医药卫生—中药学]