CPT-1基因在湘西黄牛不同组织中的发育性表达研究  

Study on the Developmental Expression of CPT-1 Gene in Different Tissues of Xiangxi Cattle

作　　者：黄胥莱 陈东[1] 万发春 沈维军[1] 易康乐 唐江权 李付强 HUANG Xu-lai;CHEN Dong;WAN Fa-chun;SHEN Wei-jun;YI Kang-le;TANG Jiang-quan;LI Fu-qiang(College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128;Hunan Institute of Animal and Veterinary Science, Changsha, Hunan 410130;Hunan Tianhua Industrial Co., Ltd, Loudi,Hunan 417000)

机构地区：[1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]湖南畜牧兽医研究所,湖南长沙410130 [3]湖南天华实业有限公司,湖南娄底417000

出　　处：《中国牛业科学》2020年第2期1-5,共5页China Cattle Science

基　　金：湖南省自然科学基金项目(2019JJ50267);湖南省教育厅科学研究项目(19A237);湖南省草食动物产业技术体系项目;现代农业(肉牛牦牛)产业技术体系建设专项资金项目(CARS-37)。

摘　　要：[目的]为了比较分析肉碱脂酰转移酶-1(carnitine palmitoyltransferase 1,CPT-1)基因在湘西黄牛不同组织中的发育性表达规律,以探讨CPT-1基因与肉牛脂肪代谢的关系。[方法]试验选取湖南德农牧业集团有限公司国家级保种场不同月龄(6月龄、18月龄和30月龄)湘西黄牛各4头,利用实时荧光定量PCR检测肝脏、背最长肌、皮下和腹腔脂肪组织CPT-1基因的相对表达量。[结果]结果表明,(1)CPT-1基因在湘西黄牛肝脏、背最长肌、皮下脂肪和腹腔脂肪的4个部位中的表达量随月龄增加依次极显著降低(P<0.01)。(2)在6月龄、30月龄时,CPT-1基因在湘西黄牛肝脏、背最长肌、皮下脂肪和腹腔脂肪的4个部位中的表达量无显著差异(P>0.05)。(3)在18月龄时,CPT-1基因在皮下脂肪中的表达量显著高于背最长肌(P<0.05),肝脏与腹腔脂肪中CPT-1基因的表达量与其他3个组织中CPT-1基因的表达量无显著差异(P>0.05)。[结论]说明CPT-1基因对湘西黄牛机体脂肪代谢具有重要作用,CPT-1基因在湘西黄牛主要脂肪代谢部位(肝脏、背最长肌、皮下脂肪和腹腔脂肪)均有表达,且CPT-1基因的表达量随月龄与部位的不同,存在月龄间差异性和组织间相似性与差异性。[Objective]The objective of study was to observe the developmental expression of carnitine palmitoyltransferase 1(CPT-1)gene in different tissues of Xiangxi cattle,and investigate the relationship between CPT-1 gene and fat metabolism in beef cattle.[Method]4 Xiangxi cattles at 6,18,and 30 months age were selected from Hunan Denong animal husbandry Co.Ltd.Each age stage was selected to extract the total RNA from liver,longest dorsal muscle,subcutaneous fat and abdominal fat.The relative mRNA expression levels of CPT-1 gene in four tissues of different ages were detected by real-time quantitative PCR.[Result]The results showed as follows:(1)The expression level of CPT-1 gene of liver,longest dorsal muscle,subcutaneous and abdominal fat of Xiangxi cattle decreased significantly with the increase of age(P<0.01).(2)At the age of 6 months and 30 months,there was no significant difference in the expression of CPT-1 gene in the four parts of liver,longissimus dorsi,subcutaneous fat and abdominal fat of Xiangxi cattle(P>0.05).(3)At 18 months of age,the expression of CPT-1 gene in subcutaneous fat was significantly higher than that of the longest dorsal muscle(P<0.05).The expression of CPT-1 gene in liver and abdominal fat was similar to that in the other three tissues.There was no significant difference in the expression of CPT-1 gene(P>0.05).[Conclusion]The results of this study indicate that the CPT-1 gene plays an important role in the lipid metabolism of Xiangxi cattle.The CPT-1 gene is expressed in the major fat metabolism sites(liver,longest back muscle,subcutaneous fat,and abdominal fat)of Xiangxi cattle,and the expression of CPT-1 gene varies with the age and location,and there are differences between months and similarities and differences between tissues.

关 键 词：CPT-1基因 湘西黄牛 部位 发育性表达 

分 类 号：S823[农业科学—畜牧学]