葡萄糖激酶调节蛋白基因rs780094多态性与冠心病痰瘀互阻证的关系探讨  

Relationship between rs780094 Polymorphism of Glucokinase Regulatory Protein Gene and Phlegm-Blood Stasis Syndrome in Coronary Heart Disease

作　　者：庞博[1] 谷春 吴敏[1] 武庆娟 邓雅文 侯雪筠[1] 叶红蕾 杨忖卿 李军[1] 刘贵建[1] PANG Bo;GU Chun;WU Min;WU Qingjuan;DENG Yawen;HOU Xueyun;YE Honglei;YANG Cunqing;LI Jun;LIU Guijian(Guang′anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中西医结合心脑血管病杂志》2020年第7期1086-1090,共5页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家中医药管理局国家中医临床研究基地业务建设科研专项课题(No.JDZX2015247)。

摘　　要：目的分析葡萄糖激酶调节蛋白基因rs780094多态性与冠心病痰瘀互阻证之间的关系。方法收集206例冠心病病人病史及实验室数据资料,提取DNA测序并进行rs780094多态性分析。依据是否为痰瘀互阻证将病人分为两组,进行统计分析。结果痰瘀互阻证冠心病病人较非痰瘀互阻证病人的血糖稍高[(6.09±2.36)mmol/L与(5.98±2.13)mmol/L,P=0.574],但未达到统计学意义。冠心病痰瘀互阻组中,rs780094 CC基因型的比例高于非痰瘀互阻证组(28.75%与14.29%,P=0.013),其等位基因C的比例也高于非痰瘀互阻证组(53.75%与39.68%,P=0.006)。Logistic回归结果显示,rs780094 CC基因型与冠心病痰瘀互阻证显著相关。结论冠心病痰瘀互阻证型与rs780094多态性存在显著关联,其分子机制有待进一步研究。Objective To analyze the relationship between rs780094 polymorphism of glucokinase regulatory protein gene and phlegm-blood stasis syndrome in coronary heart disease(CHD).Methods General population information and data from clinical lab of 206 patients with CHD were collected.Rs780094 polymorphism were analyzed by DNA sequencing of the whole blood samples.According to whether the phlegm-blood stasis syndrome existed,the patients were divided into two groups,and statistical analysis was carried out.Results The fasting blood glucose concentration in patients with phlegm-blood stasis syndrome was slightly higher than that in patients without phlegm-blood stasis syndrome[(6.09±2.36)mmol/L vs(5.98±2.13)mmol/L,P=0.574],but the difference was not significant.The proportion of rs780094 CC genotype in CHD patients with phlegm-blood stasis syndrome was significantly higher than that in CHD patients with non-phlegm-blood stasis syndrome(28.75%vs 14.29%,P=0.013).The proportion of allele C was also significantly higher than that in non-phlegm-stasis syndrome group(53.75%vs 39.68%,P=0.006).Logistic regression results showed that rs780094 CC genotype was significantly correlated with the phlegm-blood stasis syndrome of coronary heart disease.Conclusion The Phlegm-blood stasis syndrome in CHD is significantly associated with rs780094 polymorphism,and its molecular mechanism needs further study.

关 键 词：冠心病 单核苷酸多态性 痰瘀互阻证 葡萄糖激酶调节蛋白 

分 类 号：R541.4[医药卫生—心血管疾病] R256.2[医药卫生—内科学]