双色茉莉叶片初代培养物的建立及愈伤组织诱导  

Establishment of Primary Culture and Callus Induction of Brunfelsia latifolia Leaves

作　　者：马丽 郭学良[2] 周胜楠 王颖[2] 田维爽 闫世月 Ma Li;Guo Xueliang;Zhou Shengnan;Wang Ying;Tian Weishuang;Yan Shiyue(Shangqiu Normal University,Shangqiu 476000,P.R.China)

机构地区：[1]商丘师范学院生物与食品学院,商丘476000 [2]商丘师范学院,商丘476000

出　　处：《东北林业大学学报》2020年第5期67-71,共5页Journal of Northeast Forestry University

基　　金：河南省科技攻关项目(162102110092、182102110241);商丘师范学院校级青年骨干教师资助项目(2016GGJS10)。

摘　　要：以双色茉莉的叶片为外植体,探讨外植体取材时期、消毒时间、不同植物生长调节剂组合对愈伤组织诱导的影响,结果表明:取材时期对双色茉莉叶片诱导愈伤组织的影响较为显著,4月份和10月份是双色茉莉叶片取材的最佳时期;不同消毒时间对双色茉莉叶片愈伤组织诱导影响不同,其中在0.1%的氯化汞中消毒5~6 min污染率较低,且诱导产生的愈伤组织生长状况最优;培养基中6-苄氨基嘌呤(6-BA)质量浓度/萘乙酸(NAA)质量浓度影响了双色茉莉叶片愈伤组织的诱导,当6-BA质量浓度/NAA质量浓度为1/4~1时有利于愈伤组织的诱导,形成的愈伤组织质量较好;添加激动素(KT)则不利于双色茉莉叶片愈伤组织的诱导。该研究以双色茉莉叶片为外植体,培养获得了愈伤组织,其中愈伤组织诱导的适宜培养基有MS+1.0 mg·L^-16-BA+1.0 mg·L^-1 NAA、MS+1.0 mg·L^-16-BA+2.0 mg·L^-1 NAA、MS+1.0 mg·L^-16-BA+4.0 mg·L^-1 NAA和MS+2.0 mg·L^-16-BA+2.0 mg·L^-1 NAA,这为双色茉莉离体繁殖再生体系的建立奠定了基础。Taking the leaves of Brunfelsia latifolia Benth.as explants,we studied the effects of explant sampling period,disinfection time,and plant growth regulators on callus induction were discussed.The results showed that the sampling period had a great effect on callus induction from leaves of B.latifolia,and April and October were the best sampling periods.Different disinfection times have different effects on callus induction.The pollution rate of disinfection for 5-6 min in 0.1%mercuric chloride was lower,and the callus induced were in the best growth condition.The value of 6-BA/NAA in the culture medium affected the callus induction of leaves of B.latifolia.It was beneficial to the callus induction,and the callus formed was of good quality when the value of 6-BA to NAA ranged from 1/4 to 1.The concentration of KT had little effect on callus induction.The leaves of B.latifolia were used as explants,and callus was obtained by culture.The suitable medium for callus induction were MS+1 mg·L^-16-BA+1 mg·L^-1 NAA,MS+1 mg·L^-16-BA+2 mg·L^-1 NAA,MS+1 mg·L^-16-BA+4 mg·L^-1 NAA and MS+2 mg·L^-16-BA+2 mg·L^-1 NAA,which laid the foundation for establishing in vitro regeneration system of B.latifolia.

关 键 词：双色茉莉 愈伤组织 选材时期 消毒时间 植物生长调节剂 

分 类 号：S685.16[农业科学—观赏园艺] Q813.12[农业科学—园艺学]