基于药效学效果结合化学指标优选小儿汗停颗粒提取工艺  

Optimization of Extraction Process of Xiaoer Hanting Granule Based on Pharmacodynamic Effects and Chemical Indexes

作　　者：王伦 朱丽玲 邹荣 吴宁 许汉林 WANG Lun;ZHU Liling;ZOU Rong;WU Ning;XU Hanlin(College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;Wuhan Jungufang Science and Technology Development Co.,Ltd.,Wuhan 430075,China)

机构地区：[1]湖北中医药大学药学院,武汉430065 [2]武汉军谷坊科技开发有限公司,武汉430075

出　　处：《医药导报》2020年第5期686-689,共4页Herald of Medicine

摘　　要：目的:通过药效学实验和化学指标转移率,筛选小儿汗停颗粒的最佳提取工艺。方法:以各工艺所得小儿汗停颗粒,连续7 d给对应小、大剂量组别SD大鼠灌胃,末次给药后采用皮下注射毛果芸香碱建立大鼠自汗模型,对各组大鼠足跖部皮肤的汗点进行计数。同时采用高效液相色谱(HPLC)法测定黄芪甲苷和毛蕊异黄酮葡萄糖苷的含量,计算转移率。结果:各组工艺所得小儿汗停颗粒均有减少大鼠足跖部皮肤汗点的作用,全方水提工艺组和水提醇沉工艺组均能显著减少大鼠足跖部皮肤汗点(P<0.05),其中水提醇沉工艺组最显著(P<0.01)。结论:水提醇沉为小儿汗停颗粒的最佳工艺。Objective:To screen the best extraction process of Xiaoer Hanting granule and to study its pharmacodynamic effects.Methods:The SD rats of small and large dosage group received injections of Xiaoer Hanting granules for 7 days.After the last administration,the rat self-sweat model was established by subcutaneous injection of pilocarpine,and the sweat points of the plantar skin of the rats were counted.At the same time,the content of astragaloside IV and isoflavone glucoside was determined by HPLC,and the transfer rate was calculated.Results:All groups of the Xiaoer Hanting granules showed the effect of reducing the skin sweat points of the plantar part of rats.The whole water extraction process group and the water extraction and alcohol precipitation process group could significantly reduce the skin sweat points(P<0.05)of the plantar part of rats,in which the water-extracting alcohol precipitation process group was more significant(P<0.01).Conclusion:The water extracting alcohol precipitation is the best technique to Xiaoer Hanting granule.

关 键 词：小儿汗停颗粒 水提醇沉 毛果芸香碱 黄芪甲苷 毛蕊异黄酮葡萄糖苷 提取工艺 

分 类 号：R286[医药卫生—中药学] R283[医药卫生—中医学]