Comparison of the neurovascular microstructure in local skin tissues at “PC3”“PC7” and “PC9” regions in the rat  

大鼠“曲泽”,“大陵”和“中冲”穴区之间神经血管显微结构的比较

作　　者：Zhi-yun ZHANG Dong-sheng X Jian-liang ZHANG Jia WANG Jing-jing CUI Xiang-hong JNG Wan-zhu BAI 张知云;徐东升;张建梁;王佳;崔晶晶;景向红;白万柱(中国中医科学院针灸研究所,北京100700;浙江中医药大学第三临床医学院,杭州310053)

机构地区：[1]Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China [2]The Third Clinical Medical College,Zhejiang Chinese Medical University,Hangzhou 310053,China

出　　处：《World Journal of Acupuncture-Moxibustion》2020年第1期61-64,共4页世界针灸杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China Project:Nos.81774211,81801561,81774432;The Self-Selected Research Program from China Academy of Chinese Medical Sciences:Nos.ZZ13-YQ-068,ZZ201914001;National Key R and D Program of China:No.2018YFC1707804.

摘　　要：Objective:The aim of this study was to compare the microstructure of blood vessels and nerve fibers between different acupoints in the rat skin from the perspective of histology.Methods:The skin tissue at"Quze"(曲泽PC3)""Daling(大陵PC7)"and"Zhongchong(中冲PC9)"regions was taken from the rat forelimb along the pericardium meridian,and cut sagittally with a freezing microtome.After that,the skin sections were stained by fluorescent histochemistry and immunohistochemistry with phalloidin and calcitonin gene-related peptide(CGRP)to reveal the microstructure of blood vessels and nerve fibers respectively,and then examined under a laser scanning confocal microscope.Results:The microstructure of blood vessels and nerve fibers was clearly labeled with phalloidin and CGRP,respectively.The blood vessels and nerve fibers were observed in the dermis of"PC3""PC7"and"PC9"regions,where the bundle of nerve fibers distribute in parallel to the blood vessels,and sent out thin branches to surround the wall of the blood vessels.In contrast,the density of blood vessels and nerve fibers in"PC3""PC7"and"PC9"were presented orderly in an increased tendency.Conclusion:The results of present study demonstrate the differences of the neurovascular microstructure among"PC3""PC7"and"PC9"in the rat,providing a histological view to insight into the specificity of different acupoints.

关 键 词：Skin CALCITONIN gene-related peptide PHALLOIDIN The MICROSTRUCTURE of blood vessels The MICROSTRUCTURE of nerve fibers HISTOLOGY on ACUPOINTS 

分 类 号：R245[医药卫生—针灸推拿学]