高压氧对脑缺血再灌注损伤后Cx37表达及血脑屏障的影响  

Effects of hyperbaric oxygen on expression of gap junctional protein Cx37 in brain and blood brain barrier after cerebral ischemia-reperfusion in rats

作　　者：佟雪 张倩茹[1] 赵红[1] 陈学新[2] Tong Xue;Zhang Qianru;Zhao Hong(College of Basic Medical Sciences, China Medical University, Shenyang 110122, China)

机构地区：[1]中国医科大学基础医学院机能中心,沈阳110122 [2]中国医科大学附属第一临床医院高压氧科,沈阳110122

出　　处：《中国康复》2020年第5期227-231,共5页Chinese Journal of Rehabilitation

摘　　要：目的:研究高压氧(HBO)对大鼠脑缺血再灌注(CIR)损伤后缝隙连接蛋白Cx37表达及血脑屏障(BBB)通透性的影响。方法:选用240只雄性Wistar大鼠,随机分成假手术组、模型组、模型+HBO组、HBO组各60只。采用线栓法制作大鼠大脑中动脉局灶性栓塞(MCAO)模型,并于再灌注1h、9h、21h、45h、69h进入HBO舱,期间行0.25MPa HBO治疗5次。在处死动物前1h经尾静脉注射2%伊文思兰(EB),采用比色法、实时荧光定量聚合酶链反应(RT-PCR)、Western Blot法分别检测CIR后0h、4h、12h、24h、48h、72h脑组织EB的含量、Cx37mRNA及蛋白的表达变化。结果:CIR损伤后不同时间点模型组与假手术组相比较脑组织EB的含量显著增高(P<0.01),其中EB的含量以CIR损伤后4h最为明显。模型+HBO组脑组织中EB的含量与同时间的模型组相比较显著降低(P<0.01),HBO组与同时间假手术组相比较脑组织中EB的含量变化不明显。Cx37mRNA及蛋白的表达与CIR损伤后0h相比于CIR损伤后4h、12h、24h、48h、72h表达显著增高(P<0.01),以CIR损伤后24hCx37mRNA及蛋白的表达增高最明显。CIR损伤后24h模型+HBO组与模型组相比较脑组织中Cx37mRNA及蛋白的表达水平明显减少(P<0.01);CIR损伤后24hHBO组与假手术组相比脑组织中Cx37mRNA及蛋白的表达变化差异无统计学意义。结论:高压氧可以降低脑缺血再灌注后BBB通透性,其保护作用与抑制缝隙连接蛋白Cx37的表达有关。Objective:To investigate the effects of hyperbaric oxygen(HBO)on the expression of gap junctional protein Cx37 in the brain and blood brain barrier(BBB)after cerebral ischemia-reperfusion(CIR)in rats.Methods:240 male Wistar rats were randomly assigned into sham operation,model,model+HBO,and HBO groups(n=60 each).Using CIR models,0.25 MPa HBO was applied 5 times during the reperfusion period,and 2%Evens blue(EB)was injected into tail veins 1 h before the animals were executed.The expression of Cx37 mRNA and protein,and the contents of EB were determined by RT-PCR,Western blotting and spectrophotometer,respectively.Results:In model group,the EB content in brain tissue was significantly increased as compared with the sham operation group,reaching its peak at 4 h post-CIR injury(P<0.01).The expression of Cx37 mRNA and protein significantly increased consecutively in different model group as compared with the 0 h group,reaching its highest level at 24 h.There was no significant difference in the expression of Cx37 mRNA and protein,and the content of EB between HBO group and sham operation group(P>0.05).The expression of Cx37 mRNA and protein in model+HBO group at CIR 24 h was significantly decreased as compared with model group(P<0.01).Conclusion:HBO intervention can decrease the permeability of BBB via reducing the expression level of Cx37 mRNA and protein.

关 键 词：高压氧 血脑屏障 脑缺血再灌注 缝隙连接蛋白Cx37 

分 类 号：R49[医药卫生—康复医学] R743.3[医药卫生—临床医学]