基于网络药理学探究中药黄连抗菌作用机制  

Study on Anti-bacterial Mechanism of Coptidis Rhizoma Based on Network Pharmacology

作　　者：姚姗姗 刘静茹 陈静[2] 张鹏 王文静 张铁 王春光 YAO Shanshan;LIU Jingru;CHEN Jing;ZHANG Peng;WANG Wenjing;ZHANG Tie;WANG Chunguang(College of Veterinary Medicine,Traditional Chinese Veterinary Medicine,Hebei Agricultural University,Baoding 071000,China;Baoding Jizhong Pharmaceutical Co.,Ltd.,Baoding 071000,China)

机构地区：[1]河北农业大学动物医学院,中兽医学院,保定071000 [2]保定冀中药业有限公司,保定071000

出　　处：《中国畜牧兽医》2020年第5期1593-1601,共9页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31572560);河北省自然科学基金(C2018204092);河北省科技厅重点研发计划(202030504040006)。

摘　　要：试验旨在基于网络药理学方法探究中药黄连主要活性成分的抗菌作用机制。从TCMSP数据库筛选黄连有效成分及作用靶点,利用Uniprot数据库转化成相应靶点基因,将活性成分和靶点基因导入Cytoscape 3.6.1软件,构建活性成分-靶点网络图。再结合GeneCards数据库检索黄连抗菌相关靶点,将核心靶点基因上传至STRING平台,构建蛋白相互作用网络,最后,利用DAVID数据库对关键靶点进行GO生物学过程分析和KEGG通路富集分析。结果显示,黄连9种有效成分作用于105个抗菌靶点参与22条生物学过程(生物过程18条、分子功能3条、细胞组成1条)和19条相关信号通路。其中黄连的主要有效成分为槲皮素、氢化小檗碱、氧化小檗碱、小檗碱和巴马汀等;抗菌作用靶点为IL2、VCAM1、STAT1、NOS2、TGFB1、CASP9、MMP1、NFKBIA和CXCL10等;参与的生物学过程主要包括炎症反应、RNA聚合酶Ⅱ启动子转录的正调节、对脂多糖的反应、凋亡过程的负调节、细胞黏附等;主要作用于Toll样受体信号通路、肿瘤坏死因子(TNF)信号通路、NF-κB信号通路等。本试验结果表明,黄连可能通过槲皮素、小檗碱、巴马汀等活性成分作用于IL2、VCAM1、STAT1等关键靶标参与Toll样受体信号通路、TNF信号通路联合发挥抗菌作用,充分体现了黄连多成分、多靶点、多通路的抗菌作用机制,为进一步深入阐明黄连抗菌机制提供理论参考。This study was aimed to explore the antibacterial mechanism of the main components of Chinese traditional medicine Coptidis Rhizoma based on network pharmacology.The effective components and target targets of Coptidis Rhizoma were screened from the TCMSP database,and the corresponding target genes were transformed using the Uniprot database.The active components and target genes were imported into the Cytoscape 3.6.1 software to construct an active component-target network diagram.Combined with the GeneCards database to search the antibacterial related targets of Coptidis Rhizoma,the core target genes were uploaded to the STRING platform to build a protein interaction network.Finally,the DAVID database was used to analyze key targets by GO biological processes and KEGG pathway enrichment.The results of network pharmacology showed that 9 effective components of Coptidis Rhizoma acted on 105 antibacterial targets and participated in 22 biological processes(18 biological processes,3 molecular functions,1 cell composition)and 19 related signal pathways.Among them,the main active ingredients of Coptidis Rhizoma were quercetin,(R)-Canadine,berlambine,berberine and palmatine,etc.Antibacterial targets were IL2,VCAM1,STAT1,NOS2,TGFB1,CASP9,MMP1,NFKBIA and CXCL10,etc.The involved biological processes mainly included inflammatory response,positive regulation of RNA polymeraseⅡpromoter transcription,response to lipopolysaccharide,negative regulation of apoptotic process,cell adhesion,etc.Mainly act on Toll-like receptors signal pathway,TNF signal pathway,NF-kappa B signaling pathway,etc.Coptidis Rhizoma might through its active ingredients such as quercetin,berberine,palmatine act on other key targets such as IL2,VCAM1 and STAT1 to participate in the Toll-like receptor signaling pathway and TNF signaling pathway to exert antibacterial effect.It fully reflected the antibacterial action mechanism of Coptidis Rhizoma with multiple components,multiple targets and multiple pathways,and provided a theoretical reference for furth

关 键 词：网络药理学 黄连 抗菌 作用机制 

分 类 号：S853.74[农业科学—临床兽医学]