热应激对中国荷斯坦牛乳腺组织基因表达及信号通路的影响  

Effect of heat stress on gene expressions and signaling pathways of mammary gland in Chinese Holstein

作　　者：李秋玲[1] 齐颖 王琛 张一名[1] 王新妤 尚校兰[1] 贾永红[1] 李美茹[1] 储明星[2] LI Qiuling;QI Ying;WANG Chen;ZHANG Yiming;WANG Xinyu;SHANG Xiaolan;JIA Yonghong;LI Meiru;CHU Mingxing(Hebei Key Laboratory of Animal Diversity, College of Life Sciences, Langfang Normal University, Langfang Key Laboratory of Cell Engineering and Application, Langfang 065000, China;Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

机构地区：[1]廊坊师范学院生命科学学院,河北省动物多样性重点实验室,廊坊市细胞工程与应用研究重点实验室,河北廊坊065000 [2]中国农业科学院北京畜牧兽医研究所,农业农村部动物遗传育种与繁殖(家禽)重点实验室,北京100193

出　　处：《浙江农业学报》2020年第5期770-778,共9页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31402056);河北省高等学校青年拔尖人才计划(BJ2014048);农业农村部动物遗传育种与繁殖(家禽)重点实验室开放课题(poultrylab2019-8)。

摘　　要：夏季奶牛热应激问题给奶业生产造成了巨大的经济损失。为揭示奶牛发生热应激后乳腺组织应答反应的分子机制,通过研究热应激奶牛乳腺组织差异表达基因,探究差异基因对乳腺组织转录调控的影响。以8头中国荷斯坦牛为研究对象,分别采集热应激期(8月,温湿指数THI=83.8)和非热应激期(3月,THI=65.8)各4头奶牛乳腺组织,利用HiSeq2000进行转录组测序,并进行生物信息学分析,共发现96个差异表达基因(差异倍数>2),其中上调表达46个,下调表达50个。GO分类结果表明,注释到细胞组分、分子功能和生物学过程的差异基因数量分别为41、38和36个(P<0.05)。KEGG通路分析结果表明,差异基因共富集到18条信号通路(P<0.05),其中6条与疾病有关,9条与代谢有关,此外,与免疫应答相关的细胞因子-细胞因子受体相互作用和NOD样受体信号通路明显富集。通过比较热应激和非热应激奶牛乳腺组织转录组,分析差异基因相关信号通路,为进一步了解奶牛发生热应激反应的分子机制奠定基础。Heat stress caused great economic loss to dairy production in summer.To reveal the molecular mechanism of mammary gland response to heat stress,differentially expressed genes were studied to explore the effect of differentially expressed genes on transcription regulation of mammary tissues in dairy cows.Four heat stressed(August,Temperature humidity index,THI=83.8)and 4 non-heat stressed(March,THI=65.8)mammary glands of Chinese Holstein were sampled,sequenced by HiSeq2000 and analyzed by comprehensive bioinformatics.There were 96 differentially expressed genes(fold change>2)obtained,among which 46 genes were up-regulated and 50 genes were down-regulated.GO functional annotation results showed that there were 41,38 and 36 genes in cell components,molecular functions,and biological processes,respectively(P<0.05).KEGG was enriched in 18 pathways(P<0.05),including 6 pathways related to diseases,9 pathways related to metabolisms,immune response related cytokine-cytokine receptor interaction,and NOD-like receptor signaling pathway.The transcriptome of mammary gland in heat stressed and non-heat stressed Chinese Holstein were compared,and the related signaling pathways were analyzed,which may provide a theoretical basis for understanding the molecular mechanisms of heat stress in dairy cattle.

关 键 词：奶牛 热应激 乳腺 基因表达 信号通路 

分 类 号：S823[农业科学—畜牧学]