右美托咪定预先给药对罗哌卡因所致神经毒性的影响  

Effect of preadministration of Dexmetomidine on neurotoxicity induced by ropivacaine

作　　者：张金金 阳丽云[1] 庞静兰 廖龙雄 曾涟[1] Zhang Jinjin;Yang Liyun;Pang Jinglan;Liao Longxiong;Zeng Lian(Department of Anesthesiology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院麻醉科,南宁530021

出　　处：《广西医科大学学报》2020年第4期695-699,共5页Journal of Guangxi Medical University

基　　金：广西壮族自治区教育厅高校科研课题资助项目(No.YB2014082)。

摘　　要：目的:探究右美托咪定(Dex)预先给药对罗哌卡因所致大鼠肾上腺嗜铬细胞瘤细胞(PC12细胞)神经毒性的影响。方法:将PC12细胞分为6组:对照组(C组)、1μmol/L Dex+罗哌卡因组(D1R组)、10μmol/L Dex+罗哌卡因组(D10R组)、100μmol/L Dex+罗哌卡因组(D100R组)、200μmol/L Dex+罗哌卡因组(D200R组)、罗哌卡因组(R组)。D1R组、D10R组、D100R组、D200R细胞分别用1μmol/L、10μmol/L、100μmol/L、200μmol/L Dex预处理30 min,各组细胞加入罗哌卡因6mmol/L后继续培养24 h,CCK-8法检测细胞活力后;C组、D1R组、D10R组、R组4组应用倒置显微镜镜下观察细胞形态学变化;Annexin V-FITC/PI凋亡试剂盒检测凋亡细胞荧光强度;Werstern blot法检测Bax和Bcl-2蛋白相对表达水平。结果:D1R组、D10R组、D100R组细胞活力均高于R组(P<0.05),D200R组细胞活力低于R组(P<0.05);光学显微镜下C组、D10R组、D1R组、R组细胞总量依次递减,凋亡细胞比例依次增加,D10R组、D1R组的凋亡细胞相对表达量明显低于R组(P<0.05);C组、D10R组、D1R组、R组的Bax蛋白表达量、Bax/Bcl-2比率依次增加(P<0.05),C组、D10R组、D1R组、R组的Bcl-2蛋白表达量依次减少(P<0.05)。结论:Dex可以通过影响Bax/Bcl-2比率在一定剂量范围内抑制罗哌卡因所致神经毒性。Objective: To investigate the effect of dexmedetomidine(Dex)pre-administration on the neurotoxicity of ropivacaine-induced rat adrenal pheochromocytoma cells(PC12).Methods: PC12 cells were cultured and the cells in log phase were divided into 6 groups,control group(group C),1 μmol/L Dex + ropivacaine group(D1 R group),10 μmol/L Dex+ropivacaine group(D10 R Group),100 μmol/L Dex + ropivacaine group(D100 R group),200 μmol/L Dex+ropivacaine group(D200 R group),ropivacaine group(R group).D1 R group,D10 R group,D100 R group and D200 R cells were pretreated with 1 μmol/L,10 μmol/L,100 μmol/L and 200 μmol/L Dex for 30 min respectively.After adding ropivacaine 6 mmol/L,each group of cells was cultured for 24 h,After the cell viability was detected by CCK-8 method,the morphological changes of cells were observed under inverted microscope with group C,D1 R group,D10 R group and R group.The fluorescence intensity of apoptotic cells was detected by AV-FITC apoptosis kit.Western blotting method The relative expression levels of Bax,Bcl-2 protein were examined.Results: The cell viability of D1 R group,D10 R group and D100 R group was higher than that of R group(P<0.05).The cell viability of D200 R group was lower than that of R group(P<0.05).Under the light microscope,the total amount of cells in group C,D10 R group,D1 R group and R group decreased in turn,and the proportion of apoptotic cells increased in turn.The relative expression of apoptotic cells in D10 R group and D1 R group was significantly lower than that in R group(P<0.05).The expression of Bax protein in group C,D10 R group,D1 R group and R group increased significantly(P < 0.05).The expression of Bcl-2 protein in group C,D10 R group,D1 R group and R group decreased significantly(P < 0.05).Conclusion: Dex can inhibit ropivacaine-induced neurotoxicity in a certain dose range by affecting Bax/Bcl-2 ratio.

关 键 词：右美托咪定 罗哌卡因 神经毒性 

分 类 号：R96[医药卫生—药理学]