急性脑梗死患者并发肺部感染的危险因素及与IL-6基因-572C/G基因多态性相关性分析  

Risk factors of secondary pulmonary infection and the correlation with IL-6-572C/G gene polymorphism in patients with acute cerebral infarction combined with pulmonary infection

作　　者：余波 沈宇斐 陆旭东 官俏兵 罗本燕[2] YU Bo;SHEN Yu-fei;LU Xu-dong;GUAN Qiao-bing;LUO Ben-yan(Jiaxing Second Hospital,Jiaxing,Zhejiang 314000,China;不详)

机构地区：[1]嘉兴市第二医院神经内科,浙江嘉兴314000 [2]浙江大学医学院附属第一医院神经内科,浙江杭州310006

出　　处：《中华医院感染学杂志》2020年第7期999-1002,共4页Chinese Journal of Nosocomiology

基　　金：浙江省医学会临床科研基金资助项目(2016ZYC-A76)。

摘　　要：目的探讨急性脑梗死患者并发肺部感染的危险因素及与白细胞介素-6(IL-6)基因-572C/G基因多态性的关联关系。方法选择嘉兴市第二医院于2016年12月-2018年12月期间住院的急性脑梗死患者400例作为研究对象,根据是否并发肺部感染,分为肺部感染组112例与无肺部感染组288例。采用多因素Logistic回归分析影响急性脑梗死患者并发肺部感染危险因素;采用酶联免疫吸附法(Enzyme-linked immuno sorbent assay,ELISA)测定血清IL-6水平;采用限制性片段长度多态性聚合酶链反应(Polymerase chain reaction restriction fragment length polymorphism,PCR-RFLP)法检测IL-6基因-572C/G基因多态性。结果单因素分析表明,年龄、合并基础疾病、侵入性操作、吞咽功能障碍和既往肺部疾病,肺部感染率比较差异均具有统计学意义(P<0.05);多因素Logistic回归分析显示,年龄>60岁、合并基础疾病、侵入性操作和既往肺部疾病为影响肺部感染的危险因素(P<0.05);肺部感染组血清IL-6水平高于无肺部感染组(P<0.05);肺部感染组等位基因G频率高于无肺部感染组(P<0.05);肺部感染组基因型GG频率48.21%高于无肺部感染组30.21%(P<0.05)。结论年龄、合并基础疾病、侵入性操作和既往肺部疾病均为影响急性脑梗死患者并发肺部感染危险因素;急性脑梗死患者并发肺部感染IL-6基因-572G基因频率较高,且基因型GG频率较高,认为G基因可能是易感基因。OBJECTIVE To explore the risk factors of secondary pulmonary infection in patients with acute cerebral infarction combined with pulmonary infection and its correlation with interleukin-6(IL-6)gene-572 C/G polymorphism.METHODS A total of 400 patients with acute cerebral infarction hospitalized in our hospital from December 2016 to December 2018 were recruited.According to whether secondary pulmonary infection occurred,they were divided into the pulmonary infections group with 112 patients and the non-pulmonary infection groups with 288 patients.Multivariate logistic regression analysis was used to analyze the risk factors of secondary acute cerebral infarction with pulmonary infection.Serum IL-6 level was measured by ELISA and the polymorphism of IL-6-572 C/G gene was detected by PCR-RFLP method.RESULTS Univariate analysis showed that the lung infection rates in both groups were significantly different when age,underlying diseases,invasive procedures,swallowing dysfunction,and previous lung diseases were used as the variate(P<0.05).The results of logistic regression analysis showed that age>60 years old,combined with underlying diseases,invasive operation and previous lung diseases were risk factors of pulmonary infection.The level of serum IL-6 in the pulmonary infection group was significantly higher than that in the non-pulmonary infection group(P<0.05).The frequency of G allele in the pulmonary infection group was significantly higher than that in the non-pulmonary infection group(P<0.05).The frequency of genotype GG in the pulmonary infection group(48.21%)was significantly higher than that in the non-pulmonary infection group(30.21%)(P<0.05).CONCLUSION Age,combined with basic diseases,invasive manipulation and previous pulmonary diseases were risk factors of secondary acute cerebral infarction combined with pulmonary infection.The frequency of IL-6-572 G gene and genotype GG in secondary acute cerebral infarction with pulmonary infection was higher,suggesting that G may be a susceptible gene polymorphism site

关 键 词：急性脑梗死 肺部感染 危险因素 IL-6基因-572C/G基因 

分 类 号：R563.1[医药卫生—呼吸系统]