复方甘草酸苷调控SDF-1/CXCR-4轴治疗特应性皮炎小鼠的作用机制  

Mechanisms of Compound Glycyrrhizin Regulating SDF-1/CXCR-4 Axis in the Treatment of Atopic Dermatitis in Mice

作　　者：蔡夏叶 徐恩国 唐梦丹[1] 王翔[1] Cai Xiaye;Xu Enguo;Tang Mengdan;Wang Xiang(Department of Dermatology,Taizhou First People's Hospital,Zhejiang Taizhou 318020,China;Department of Cardiology,Taizhou First People's Hospital,Zhejiang Taizhou 318020,China)

机构地区：[1]台州市第一人民医院皮肤科,浙江台州318020 [2]台州市第一人民医院心内科,浙江台州318020

出　　处：《中国药师》2020年第4期638-642,共5页China Pharmacist

摘　　要：目的:探讨复方甘草酸苷调控基质细胞衍生因子-1/趋化因子受体-4(SDF-1/CXCR-4)轴治疗特应性皮炎小鼠的作用机制。方法:120只Nc/Nga小鼠随机分成6组:正常对照组、模型组、复方甘草酸苷低(10 mg·kg^-1)、中(20 mg·kg^-1)、高(40 mg·kg^-1)剂量组、地塞米松组(30 mg·kg^-1),每组20只。模型组、地塞米松组、复方甘草酸苷各剂量组建立特应性皮炎模型。造模成功24 h后地塞米松组、复方甘草酸苷各剂量组分别灌胃给予相应药物,1次/d,连续给药14 d,正常对照组及模型组给予等体积生理盐水。试验结束后,游标卡尺测量小鼠右耳厚度,实时荧光逆转录法(RT-PCR)法及蛋白质免疫印迹(Western-blot)法测定右耳组织SDF-1、CXCR-4 mRNA及蛋白表达水平。结果:模型组右耳厚度、耳部皮炎组织SDF-1、CXCR-4 mRNA及蛋白表达水平显著高于正常对照组(P<0.05);地塞米松组、复方甘草酸苷各剂量组右耳厚度、耳部皮炎组织SDF-1、CXCR-4 mRNA及蛋白表达水平显著低于模型组(P<0.05),且随着复方甘草酸苷给药剂量的增加,各指标呈下降趋势(P<0.05);复方甘草酸苷低、中剂量组各指标与地塞米松组差异有统计学意义(P<0.05)。模型组耳部皮肤结构缺失,细胞水肿明显,大量淋巴细胞浸润;地塞米松组及复方甘草酸苷高剂量组皮肤结构较为完整,细胞水肿及炎细胞浸润明显减少;复方甘草酸苷低、中剂量组皮肤结构仍有缺失,细胞水肿、淋巴细胞浸润较模型组减轻。结论:复方甘草酸苷能明显抑制小鼠耳部特应皮炎反应、抑制小鼠耳部炎症细胞浸润;其机制与复方甘草酸苷能抑制SDF-1、CXCR-4 mRNA及蛋白表达水平有关。Objective: To investigate the mechanisms of compound glycyrrhizin regulating stromal cell-derived factor-1/chemokine receptor 4( SDF-1/CXCR-4) Axis in the treatment of atopic dermatitis in mice. Methods: Totally 120 Nc/Nga mice were randomly divided into six groups: the normal control group,the model group,compound glycyrrhizin low( 10 mg · kg^-1),medium( 20 mg·kg^-1) and high( 40 mg·kg^-1) dose groups,and dexamethasone group( 30 mg·kg^-1) with 20 ones in each group. The mice in the model group,dexamethasone group and compound glycyrrhizin three dose groups were used to establish atopic dermatitis model. At 24 h after successful modeling,the mice in dexamethasone group and compound glycyrrhizin dose groups were given corresponding drug by gavage for 14 consecutive days( once a day),while the mice in the normal control group and the model group were given the same volume of saline. At the end of the experiment,the thickness of right ear was measured by vernier caliper,and SDF-1,CXCR-4 mRNA and protein expressions were measured by RT-PCR and Western blot. Results: The thickness of right ear and the expression levels of SDF-1,CXCR-4 mRNA and protein in dermatitis tissue of the model group were significantly higher than those of the normal control group( P<0.05). The thickness of right ear and the expression levels of SDF-1,CXCR-4 mRNA and protein in dermatitis tissue of dexamethasone group and compound glycyrrhizin dose groups were significantly lower than those of the model group( P< 0.05). With the dose increase of compound glycyrrhizin,each index showed a downward trend( P<0.05). There were significant differences in the indices between compound glycyrrhizin low and medium dose groups and dexamethasone group( P< 0.05). In the model group,the skin structure was absent with obvious cell edema and a large number of lymphocyte infiltration. In dexamethasone group and ompound glycyrrhizin high dose group,the skin structure was relatively complete with obvious decrease of cell edema and inflammatory cell infiltrati

关 键 词：复方甘草酸苷 基质细胞衍生因子-1/趋化因子受体-4 特应性皮炎 

分 类 号：R965.1[医药卫生—药理学]