基于trnL-trnF基因序列鉴定10种枸杞属种质资源  

Identifying 10 Lycium L.Plants Based on trnL-trnF Sequence as Barcodes

作　　者：万如 王亚军 赵建华 马婷慧[2] 石志刚[2] Wan Ru;Wang Yajun;Zhao Jianhua;Ma Tinghui;Shi Zhigang(Wolfberry Engineering Institute,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002;Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002)

机构地区：[1]宁夏农林科学院枸杞工程技术研究所,宁夏银川750002 [2]宁夏农林科学院,宁夏银川750002

出　　处：《宁夏农林科技》2020年第1期14-15,共2页Journal of Ningxia Agriculture and Forestry Science and Technology

基　　金：国家自然科学基金项目(31360471);宁夏回族自治区育种专项(2013NYYZ0106);宁夏回族自治区科技创新领军人才专项(KJT2015014);宁夏回族自治区全产业链创新示范项目(QCYL-2018-05);宁夏回族自治区科技创新引导基础研究项目(NKYJ-18-02);宁夏农林科学院成果转化项目(NNKZZCGZH-2018-15)。

摘　　要：通过对10份枸杞属种质材料的叶绿体基因trnL-trnF进行克隆测序,获得10条DNA序列,经过比对分析及构建系统发育树,得出以下结果:①trnL-trnF序列总长为1156 bp,其中保守位点1146个,变异位点10个,包括简约信息位点9个,自裔位点1个;GC含量均值为35.9%;碱基转换颠换值为4.4。②获得的系统发育树分成了两大支,即黑果、W-12-30、黄果变和W-12-27聚为一大支,其余品种聚为一支,各分支内部均具有较高的自展支持率。因此,trnL-trnF序列可以作为初步鉴定枸杞属植物的DNA条形码。Through cloning for sequencing the chloroplast genes trnL-trnF from 10 germplasm materials of Lycium L.,10 DNA sequences were obtained.After alignment analysis and building phylogenetic tree,the results showed that①the total length of trnL-trnF sequence was 1156 bp,which included 1146 conserved sites,10 variable sites,9 PI-parsimony-informativesites and a C-conservedsite.The mean value of GC content was 35.9%and the R value was 4.4.②The MP tree divided 10 test materials into two parts.Heiguo,W-12-30,Huangguobian and W-12-27 plants were divided into one branch.Others were in one branch.High favorability rating of bootstrap was showed in two parts.Therefore,trnL-trnF sequence could be the DNA barcode to preliminarily distinct Lycium L.plants.

关 键 词：枸杞 trnL-trnF基因 DNA条形码 分子鉴定 

分 类 号：S567.19[农业科学—中草药栽培]