山茶油中原花青素的提取工艺优化及含量测定  

Optimization of Extraction Technology and Determination of Procyanidins from Camellia Oil

作　　者：王宗成 路大中[1] 王守敏 郑辉煌 杨丽华 罗小芳 Wang Zongcheng;Lu Dazhong;Wang Shoumin;Zheng Huihuang;Yang Lihua;Luo Xiaofang(College of chemical and biological engineering,Hunan University of Science and Engineering,Yongzhou 425199;Hunan Provincial Engineering Research Center for Comprehensive Development and Utilization of Biomass Resources,Hunan University of Science and Engineering,Yongzhou 425199)

机构地区：[1]湖南科技学院化学与生物工程学院,永州425199 [2]湖南科技学院湖南省生物质资源综合开发利用工程技术研究中心,永州425199

出　　处：《中国粮油学报》2020年第4期61-65,70,共6页Journal of the Chinese Cereals and Oils Association

基　　金：湖南省自然科学基金项目(2018JJ3196);湖南省普通高校校企合作创新创业教育基地资助(2018-380);湖南省大学生创新创业训练计划项目(2019-219);湖南省高校科技创新团队支持计划(2012-318)。

摘　　要：优化山茶油中原花青素的提取工艺,并建立其含量测定方法。以原花青素标准品为对照品,采用紫外分光光度法测定山茶油中原花青素的含量。通过单因素试验和正交试验相结合的方法考察甲醇浓度、提取温度、提取时间和提取次数对山茶油中原花青素提取的影响。山茶油中原花青素最佳提取工艺为甲醇体积分数50%、提取温度80℃、提取时间40 min、提取4次;在此条件下所提取得到山茶油中平均原花青素含量为265.90 mg/kg。山茶油中含有较高含量的原花青素,所得的优选工艺稳定可行,原花青素含量测定方法可靠。To optimize the extraction technology of procyanidins from camellia oil and establish its content determination method.The content of procyanidins in camellia oil was determined by UV spectrophotometry with the standard procyanidins as the reference.The effects of methanol concentration,extraction temperature,extraction time and extraction times on the extraction of procyanidins from camellia oil were investigated by single factor test and orthogonal test.The optimal extraction technology conditions of procyanidins in camellia oil were as follows:methanol volume fraction 50%,water-bath temperature 80℃,extraction time 40 min and extraction for 4 times.Under these conditions,the average content of procyanidins in camellia oil was 265.90 mg/kg.Camellia oil contained high content of procyanidins,the optimized technology was stable and feasible,and the method for determination of procyanidins was reliable.

关 键 词：山茶油 原花青素 紫外分光光度法 含量测定 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]